Abstract | PURPOSE: MATERIALS AND METHODS: Normal, cancerous and metastatic gastric tissues were collected from patients diagnosed with advanced gastric cancer. The in vivo expression of periostin was evaluated by in situ hybridization and immunofluorescent staining. Meanwhile, human gastric adenocarcinoma cell lines MKN-45 and BGC-803 were used to detect the in vitro expression of periostin by using quantitative real-time polymerase chain reaction (PCR) and western blotting. RESULTS:
Periostin is expressed in the stroma of the primary gastric tumors and metastases, but not in normal gastric tissue. In addition, we observed that periostin is located mainly in pericryptal fibroblasts, but not in the tumor cells, and strongly correlated to the expression of α-smooth muscle actin (SMA). Furthermore, the distribution patterns of periostin were broader as the clinical staging of tumors progressed. We also identified a role of stress-related signaling in promoting cancer development and progression, and found that isoprenaline upregulated expression levels of periostin in gastric cancer cells. CONCLUSION:
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Authors | Guo-Xiao Liu, Hong-Qing Xi, Xiao-Yan Sun, Zhi-Jun Geng, Shao-Wei Yang, Yan-Jie Lu, Bo Wei, Lin Chen |
Journal | Yonsei medical journal
(Yonsei Med J)
Vol. 57
Issue 3
Pg. 557-64
(May 2016)
ISSN: 1976-2437 [Electronic] Korea (South) |
PMID | 26996552
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adrenergic beta-Agonists
- Cell Adhesion Molecules
- POSTN protein, human
- RNA, Messenger
- Isoproterenol
|
Topics |
- Adenocarcinoma
(metabolism, pathology)
- Adrenergic beta-Agonists
(pharmacology)
- Aged
- Blotting, Western
- Cell Adhesion Molecules
(drug effects, metabolism)
- Cell Line, Tumor
- Fibroblasts
(metabolism)
- Gastric Mucosa
(metabolism)
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Isoproterenol
(pharmacology)
- Male
- Neoplasm Staging
- RNA, Messenger
(genetics, metabolism)
- Real-Time Polymerase Chain Reaction
- Signal Transduction
- Stomach
(pathology)
- Stomach Neoplasms
(metabolism, pathology)
- Up-Regulation
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