Extracts of Bauhinia championii (Benth.) Benth. inhibit NF-<kappa>B-signaling in a rat model of collagen-induced arthritis and primary synovial cells.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Bauhinia championii (Benth.) Benth. is used in Chinese traditional medicine to treat arthritis, especially has been used a long time ago on rheumatoid arthritis (RA) in She ethnic minority group.

AIM OF THE RESEARCH:

To investigate the anti-RA effect of Bauhinia championii (Benth.) Benth ethyl acetate extract (BCBEE) and the molecular bases of it.

MATERIALS AND METHODS:

BCBEE was studied on a rat model of RA induced by Ⅱcollagen in vivo, as well as on primary synovial cells in vitro.

RESULTS:

After BCBEE treatment, in vivo, it was showed that paw and joint edema was inhibited, pathological joint changes was ameliorated and the levels of interleukin (IL)-1β and tumor necrosis factor-<alpha>(TNF-α) was decreased significantly. The protein and mRNA expressions of nuclear factor-<kappa>B (NF-κB)(p65), IκB, p-IκB and IκB kinase beta (IκKβ) were also down-regulated. Moreover, the in vitro study revealed that BCBEE treatment inhibited primary synovial cells proliferation, and promoted down-regulation of NF-κB(p65), IκB, p-IκB and IκKβ.

CONCLUSIONS:

Taken together, the present study demonstrates that BCBEE produces a protection in a rat model of RA induced by Ⅱcollagen via inhibiting paw and joint edema, ameliorating pathological joint changes and regulating the levels of cytokines and its action mechanism maybe is via down-regulating NF-κB(p65), IκB, p-IκB and IκKβ expression.

• Authors: Wei Xu, Mingqing Huang, Yuqin Zhang, Huang Li, Haiyin Zheng, Lishuang Yu, Kedan Chu
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 185 Pg. 140-6 (Jun 05 2016) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 26988564 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Cytokines
• Plant Extracts
• Collagen

Topics

• Animals
• Arthritis (chemically induced, drug therapy)
• Bauhinia (chemistry)
• Collagen (toxicity)
• Cytokines (genetics, metabolism)
• Female
• Gene Expression Regulation (drug effects)
• Male
• Mice
• Plant Extracts (chemistry, pharmacology)
• Rats
• Rats, Wistar
• Signal Transduction (drug effects)
• Synovial Membrane (drug effects)