Evaluation of Vascular Endothelial Growth Factor and Its Receptors in Human Neointima.

Abstract

OBJECTIVE:

The potential contribution of vascular endothelial growth factor (VEGF) in neointima development has been evaluated in numerous animal studies. However, its role remains controversial. Moreover, little is known about neointima formation in humans. In this study we assessed the expression of VEGF-A and its receptors in the human neointima formed within vascular anastomosis.

METHODS:

The studied material comprised neointima samples harvested during secondary vascular operations from patients with chronic limb ischemia after aorto-/iliofemoral bypass grafting who developed vascular graft occlusion at 6-18 months after the initial surgical treatment. The control material consisted of segments of femoral arteries without visible macroscopic lesions collected from organ donors. The expression and content of VEGF-A, VEGFR-1 and VEGFR-2 were analyzed with PCR and ELISA methods, respectively.

RESULTS:

We observed a significantly increased expression of VEGF-A and VEGFR-2 mRNA in neointima compared to the normal aorta. A significantly higher protein content of VEGF-A and VEGFR-2 in neointima samples compared to the controls was also observed. No significant difference of VEGFR-1 content and VEGFR-1 mRNA expression was found in the studied material.

CONCLUSION:

These results indicate a possible involvement of the VEGF-A and VEGFR-2 system in the pathologic process of human neointima formation after vascular interventions.

• Authors: Marta Bruczko, Małgorzata Wolańska, Andrzej Małkowski, Krzysztof Sobolewski, Radosław Kowalewski
• Journal: Pathobiology : journal of immunopathology, molecular and cellular biology (Pathobiology) Vol. 83 Issue 1 Pg. 47-52 ( 2016) ISSN: 1423-0291 [Electronic] Switzerland
• PMID: 26890264 (Publication Type: Evaluation Study, Journal Article)
• Copyright: © 2016 S. Karger AG, Basel.

Chemical References

• RNA, Messenger
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• FLT1 protein, human
• KDR protein, human
• Vascular Endothelial Growth Factor Receptor-1
• Vascular Endothelial Growth Factor Receptor-2

Topics

• Enzyme-Linked Immunosorbent Assay
• Female
• Gene Expression
• Graft Occlusion, Vascular
• Humans
• Male
• Middle Aged
• Muscle, Smooth, Vascular (cytology)
• Neointima (genetics, physiopathology)
• Neovascularization, Pathologic
• Polymerase Chain Reaction
• RNA, Messenger (genetics)
• Vascular Endothelial Growth Factor A (genetics, metabolism)
• Vascular Endothelial Growth Factor Receptor-1 (genetics, metabolism)
• Vascular Endothelial Growth Factor Receptor-2 (genetics, metabolism)