Abstract | BACKGROUND: METHODS: Twenty-four mice were randomly allocated to normal control group (n = 6), blank control group (n = 6), VEGF gene transfection group (n = 6), and NGF gene transfection group (n = 6). The model of left hindlimb ischemia model was established by ligating the femoral artery. VEGF165plasmid (125 μg) and NGF plasmid (125 μg) was injected into the ischemic gastrocnemius of mice from VEGF group and NGF group, respectively. Left hindlimb function and ischemic damage were assessed with terminal points at 21th day postischemia induction. The gastrocnemius of four groups was tested by hematoxylin- eosin staining, proliferating cell nuclear antigen and CD34 immunohistochemistry staining, and myosin ATPase staining. NGF and VEGF protein expression was detected by enzyme-linked immunosorbent assay. RESULTS: On the 21th day after surgery, the functional assessment score and skeletal muscle atrophy degree of VEGF group and NGF group were significantly lower than those of normal control group and blank control group. The endothelial cell proliferation index and the capillary density of VEGF group and NGF group were significantly increased compared with normal control group and blank control group (P < 0.05). The NGF and VEGF protein expression of NGF group showed a significant rise when compared with blank control group (P < 0.05). Similarly, the VEGF protein expression of VEGF group was significantly higher than that of blank control group (P < 0.05), but there was no significant difference of the NGF protein expression between VEGF group and blank control group (P > 0.05). The type I skeletal muscle fiber proportion in gastrocnemius of NGF group and VEGF group was significantly higher than that of blank control group (P < 0.05). CONCLUSIONS:
NGF transfection can promote NGF and VEGF protein expression which not only can induce angiogenesis but also induce type I muscle fiber expression in ischemic limbs.
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Authors | Yong-Peng Diao, Feng-Kui Cui, Sheng Yan, Zuo-Guan Chen, Li-Shan Lian, Li-Long Guo, Yong-Jun Li |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 129
Issue 3
Pg. 313-9
(Feb 05 2016)
ISSN: 2542-5641 [Electronic] China |
PMID | 26831234
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD34
- Vascular Endothelial Growth Factor A
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Topics |
- Animals
- Antigens, CD34
(metabolism)
- Female
- Hindlimb
(metabolism, pathology)
- Ischemia
(metabolism, pathology)
- Mice
- Muscle, Skeletal
(metabolism, pathology)
- Neovascularization, Physiologic
(genetics, physiology)
- Random Allocation
- Vascular Endothelial Growth Factor A
(genetics, physiology)
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