Decreased Expression of Caveolin-1 and E-Cadherin Correlates with the Clinicopathologic Features of Gastric Cancer and the EMT Process.

Abstract

BACKGROUND:

Recent researches and relevant patents have been reported to prove the significant value of Caveolin-1 (Cav-1) in cancer diagnosis and treatment.

OBJECTIVE:

Our study aimed to study the role of Cav-1 in gastric cancer progression and investigate the relationship between Cav-1/E-cadherin expression and the clinical status of gastric cancer.

METHOD:

Immunohistochemistry was applied to detect the expression of Cav-1 and E-cadherin in gastric cancer in a tissue microarray. Real-time PCR was used to further detect the mRNA expression of Cav-1 and E-cadherin in tumor-derived and peritumoral tissues and in different gastric cancer cell lines. The expression of E-cadherin was analyzed by Western Blot and the cell migration ability was examined by Transwell migration assays after downregulation of Cav-1 using siRNA.

RESULTS:

The staining of Cav-1 and E-cadherin were both strong in all 5 of the normal gastric tissues, while in gastric cancer tissues the staining of Cav-1 and E-cadherin were downregulated (44&33 negative, 21&22weak and 5&15 strong). And their level was correlated with tumor clinical stage, pathological grade, and metastasis status. Moreover, there was a positive correlation between Cav-1 expression and E-cadherin expression in gastric cancer tissues(r = 0.42, P < 0.05). Knockdown of Cav-1 resulted in decreased expression of E-cadherin, cell morphology changes and elevated migration ability of gastric cancer cells.

CONCLUSION:

Decreased expression of Cav-1 and E-cadherin may play an important role in the progression of gastric cancer; Knockdown of Cav-1 may promote EMT of gastric cancer by targeting E-cadherin.

• Authors: Kundong Zhang, Guang Yang, Weidong Wu, Jing Zhang, Xiang Xia, Tao Jiang, Jun Cao, Kejan Huang, Zhengjun Qiu, Chen Huang
• Journal: Recent patents on anti-cancer drug discovery (Recent Pat Anticancer Drug Discov) Vol. 11 Issue 2 Pg. 236-44 ( 2016) ISSN: 2212-3970 [Electronic] United Arab Emirates
• PMID: 26817615 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• CAV1 protein, human
• CDH1 protein, human
• Cadherins
• Caveolin 1

Topics

• Antigens, CD
• Cadherins (biosynthesis, genetics)
• Caveolin 1 (biosynthesis, genetics)
• Cell Line, Tumor
• Epithelial-Mesenchymal Transition (physiology)
• Gene Expression Regulation, Neoplastic
• Humans
• Single-Blind Method
• Stomach Neoplasms (genetics, metabolism)