The Features of Inflammation Factors Concentrations in Aqueous Humor of Polypoidal Choroidal Vasculopathy.

Abstract

PURPOSE:

To investigate the cytokine concentrations in the aqueous humor of patients with refractory polypoidal choroidal vasculopathy (PCV).

METHODS:

Three separate groups of patients were studied-refractory PCV (Group A, 41 eyes), stable PCV (Group B, 39 eyes) and senile cataract (Group C, 44 eyes). Aqueous humor samples were collected at two time points for Groups A and B-before the first intravitreal ranibizumab injection and before the last injection. Aqueous humor samples were collected prior to phacoemulsification in Group C. The cytokine concentrations of interleukin 2, 6, and 8 (IL-2, IL-6, and IL-8), tumor necrosis factor α (TNF-α), monocyte chemotactic protein 1 (MCP-1), and vascular endothelial growth factor (VEGF) were measured by cytometric bead array and flow cytometry.

RESULTS:

Before the first treatment, the MCP-1, VEGF, and TNF-α levels in Group A were significantly higher than those in Group C (P < 0.05), and the MCP-1 and VEGF levels in Group A were significantly higher than those in Group B (P < 0.05). Significantly higher MCP-1 and VEGF levels were seen in Group B compared to Group C (P < 0.05). Before the final treatment, the MCP-1, VEGF, and TNF-α concentrations in Group A were significantly higher than those in Group B (P < 0.05) and Group C (P < 0.05). IL-2 levels were significantly lower in Group A compared to Group B (P < 0.05) and Group C (P < 0.05).

CONCLUSION:

Inflammatory cytokines such as MCP-1, VEGF, and TNF-α may be associated with the pathogenesis of both stable and refractory PCV.

• Authors: Jie Hu, Xuan Leng, Yijun Hu, Alp Atik, Xin Song, Zhixi Li, Yuhua Liu, Lin Lu
• Journal: PloS one (PLoS One) Vol. 11 Issue 1 Pg. e0147346 ( 2016) ISSN: 1932-6203 [Electronic] United States
• PMID: 26799405 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenesis Inhibitors
• CCL2 protein, human
• Chemokine CCL2
• Tumor Necrosis Factor-alpha
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• Ranibizumab

Topics

• Angiogenesis Inhibitors (therapeutic use)
• Aqueous Humor (metabolism)
• Chemokine CCL2 (metabolism)
• China
• Choroid (blood supply, immunology, pathology)
• Choroiditis (pathology)
• Female
• Humans
• Inflammation (pathology)
• Intravitreal Injections
• Male
• Middle Aged
• Ranibizumab (therapeutic use)
• Retinal Pigment Epithelium (blood supply, pathology)
• Retrospective Studies
• Tumor Necrosis Factor-alpha (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)