Abstract | BACKGROUND: METHOD: We analyzed the biological characteristics of FH, then utilized reverse transcription-polymerase chain reaction (RT-PCR) to study FH mRNA expression in A549 and 16 human bronchial epithelial (HBE) cell lines. The protein expression of FH was detected in 57 cases of human lung cancer tissues and 19 cases of normal lung tissues by immunohistochemistry. RESULTS: 1. Bioinformatic analysis: FH mainly exist in the mitochondria; the common structural elements of FH are mainly α-helix, random coil, β-turn, and extended strand; there are five possible transmembrane domains in the entire polypeptide chain; FH is a hydrophilic and soluble protein. 2. RT-PCR result: FH mRNA expression was downregulated in A549 cells compared with 16HBE cells. 3. Immunohistochemistry: FH protein expression was significantly lower in lung cancer cells than in normal lung tissues (P < 0.05), but was not correlated with the patients' age, gender, tumor size, pathological type, or lymph node, distant, or tumor node metastasis stage. CONCLUSION:
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Authors | Zongjuan Ming, Meihua Jiang, Wei Li, Na Fan, Wenjing Deng, Yujie Zhong, Yuping Zhang, Qiuhong Zhang, Shuanying Yang |
Journal | Thoracic cancer
(Thorac Cancer)
Vol. 5
Issue 6
Pg. 543-9
(Nov 2014)
ISSN: 1759-7706 [Print] Singapore |
PMID | 26767050
(Publication Type: Journal Article)
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