Abstract |
Coexistence of hepatitis B surface antigen ( HBsAg) and antibody against HBsAg (anti-HBs) comprises an atypical serological profile in patients with chronic hepatitis B virus (HBV) infection. In this study, in total 94 patients with coexisting HBsAg and anti-HBs and 94 age- and sex-matched patients with positive HBsAg were characterized by quantitatively measuring HBsAg and HBV DNA, sequencing large S genes, and observing clinical features. Compared with common hepatitis B patients, the patients with coexisting HBsAg and anti-HBs had lower HBsAg and HBV DNA levels. These two groups had similar rate of pre-S deletion mutations. However, in patients with coexisting HBsAg and anti-HBs, more amino acid substitutions in the a determinant of S gene were observed in HBV genotype C, but not in genotype B. Fourteen patients with coexisting HBsAg and anti-HBs were followed up for an average of 15.5 months. There were no significant changes in the levels of HBsAg, anti-HBs, HBV DNA and ALT over the follow-up period. Compared with the baseline sequences, amino acid substitutions in the MHR of HBsAg occurred in 14.3% (2/14) patients. In conclusion, coexistence of HBsAg and anti-HBs may be associated with higher frequency of mutations in the a determinant of HBV genotype C.
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Authors | Yong Liu, Le Zhang, Jin-Yong Zhou, Jinshun Pan, Wei Hu, Yi-Hua Zhou |
Journal | PloS one
(PLoS One)
Vol. 11
Issue 1
Pg. e0146980
( 2016)
ISSN: 1932-6203 [Electronic] United States |
PMID | 26751075
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Viral
- Hepatitis B Antibodies
- Hepatitis B Surface Antigens
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Amino Acid Sequence
- Amino Acid Substitution
- DNA, Viral
(genetics)
- Enzyme-Linked Immunosorbent Assay
- Female
- Follow-Up Studies
- Gene Deletion
- Genotype
- Hepatitis B Antibodies
(blood, immunology)
- Hepatitis B Surface Antigens
(blood, immunology)
- Hepatitis B virus
(genetics)
- Hepatitis B, Chronic
(immunology, virology)
- Humans
- Male
- Middle Aged
- Molecular Sequence Data
- Protein Structure, Tertiary
- Sequence Analysis, DNA
- Sequence Deletion
- Young Adult
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