miR-193a-3p regulation of chemoradiation resistance in oesophageal cancer cells via the PSEN1 gene.

Abstract

Chemoradiation therapy is an important component of the curative treatment for oesophageal carcinomas. These therapeutic effects are prevented in patients according to radioresistance and multi-drug resistance, and the cause of such resistance remains unclear. In this study, we identified the role of miR-193a-3p in modulating the radioresistance and chemoresistance of oesophageal cancer cells. We found that KYSE150 and KYSE410 cells could be characterized as relatively radiation-sensitive and radiation-resistant cells, respectively. Similarly, KYSE150 and KYSE410 cells were found to be chemosensitive and chemoresistant, respectively. Over-expression of miR-193a-3p increased the radioresistance and chemoresistance of oesophageal squamous cell carcinoma (ESCC) cells. In contrast, the down-regulation of miR-193a-3p decreased the radioresistance and chemoresistance of ESCC cells. In addition, miR-193a-3p inducing DNA damage has also been demonstrated through measuring the level of gamma-H2AX associated with miR-193a-3p. Moreover, a small interfering RNA(siRNA)-induced repression of the PSEN1 gene had an effect similar to that of miR-193a-3p up-regulation. The above processes also inhibited oesophageal cancer cells apoptosis. These findings suggest that miR-193a-3p contributes to the radiation and chemotherapy resistance of oesophageal carcinoma by down-regulating PSEN1. Thus, miR-193a-3p and PSEN1 might be potential biomarkers for chemoradiation resistant cancers.

• Authors: Fang Meng, Liting Qian, Lei Lv, Bojin Ding, Gieping Zhou, Xu Cheng, Sanqiang Niu, Yu Liang
• Journal: Gene (Gene) Vol. 579 Issue 2 Pg. 139-45 (Apr 01 2016) ISSN: 1879-0038 [Electronic] Netherlands
• PMID: 26743123 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• MIRN193 microRNA, human
• MicroRNAs
• PSEN1 protein, human
• Presenilin-1

Topics

• Apoptosis (drug effects, radiation effects)
• Cell Line, Tumor
• Chemoradiotherapy
• DNA Damage (drug effects, radiation effects)
• Drug Resistance, Neoplasm (genetics)
• Esophageal Neoplasms (drug therapy, genetics, radiotherapy)
• Gene Expression Regulation, Neoplastic (drug effects, radiation effects)
• Humans
• MicroRNAs (biosynthesis, genetics)
• Presenilin-1 (biosynthesis, genetics)
• Xenograft Model Antitumor Assays