IL-17A up-regulates expression of endothelial tissue factor in liver cirrhosis via the ROS/p38 signal pathway.

Abstract

Interleukin-17A (IL-17A), an inflammatory cytokine, is elevated in liver cirrhosis. Inflammation and coagulation dysfunction are closely related. Tissue factor (TF) is a bridge between endothelial activation, blood coagulation and inflammation. The aims of the present study were to evaluate endothelial TF expression in liver cirrhosis and identify the possible underlying role of IL-17A in TF expression. In the present study, we found that TF expression was increased on endothelium of splenic vein from cirrhotic patients and significantly correlated with intima/media ratios of splenic vein and coagulation parameters. Serum levels of IL-17A were significantly higher in cirrhotic patients as compared with normal controls. Cirrhotic serum and IL-17A stimulated TF expression in HUVECs, which was reduced by blockade of IL-17A, p38, and reactive oxygen species (ROS). Taken together, our data show that enhanced expression of endothelial TF, which plays an important role in coagulopathy and splenic vein remodeling in liver cirrhosis, is induced by IL-17A in a ROS dependent manner.

• Authors: Yansong Pu, Shu Zhang, Rui Zhou, Na Huang, Han Li, Wei Wei, Liang Li, Chen Huang, Jun Yang, Zongfang Li
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 470 Issue 1 Pg. 41-47 (Jan 29 2016) ISSN: 1090-2104 [Electronic] United States
• PMID: 26742425 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• IL17A protein, human
• Interleukin-17
• Reactive Oxygen Species
• Thromboplastin
• p38 Mitogen-Activated Protein Kinases

Topics

• Endothelium, Vascular (metabolism)
• Gene Expression Regulation
• Humans
• Interleukin-17 (metabolism)
• Liver Cirrhosis (metabolism)
• Reactive Oxygen Species (metabolism)
• Signal Transduction
• Splenic Vein (metabolism)
• Thromboplastin (metabolism)
• Up-Regulation
• p38 Mitogen-Activated Protein Kinases (metabolism)