O-linked β-N-acetylglucosamine modification of proteins is activated in post-ischemic brains of young but not aged mice: Implications for impaired functional recovery from ischemic stress.

Abstract

To evaluate the effect of age on the response of brains to an ischemic challenge, we subjected young and aged mice to transient forebrain ischemia, and analyzed the heat shock response and unfolded protein response, ubiquitin conjugation and SUMO conjugation, and O-linked β-N-acetylglucosamine modification of proteins (O-GlcNAcylation). The most prominent age-related difference was an inability of aged mice to activate O-GlcNAcylation. Considering many reports on the protective role of O-GlcNAcylation in various stress conditions including myocardial ischemia, this pathway could be a promising target for therapeutic intervention to improve functional recovery of aged patients following brain ischemia.

• Authors: Shuai Liu, Huaxin Sheng, Zhui Yu, Wulf Paschen, Wei Yang
• Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (J Cereb Blood Flow Metab) Vol. 36 Issue 2 Pg. 393-8 (Feb 2016) ISSN: 1559-7016 [Electronic] United States
• PMID: 26661187 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2015.

Chemical References

• SUMO-1 Protein
• Acetylglucosamine

Topics

• Acetylglucosamine (metabolism)
• Aging (pathology)
• Animals
• Brain (pathology)
• Brain Ischemia (genetics, metabolism, pathology, psychology)
• Male
• Mice
• Mice, Inbred C57BL
• Myocardial Ischemia (genetics, physiopathology)
• Protein Processing, Post-Translational (genetics)
• Psychomotor Performance
• Recovery of Function
• SUMO-1 Protein (metabolism)
• Stress, Physiological (genetics)
• Stroke (pathology, psychology)
• Ubiquitination
• Unfolded Protein Response (genetics)