An anthraquinone derivative from Luffa acutangula induces apoptosis in human lung cancer cell line NCI-H460 through p53-dependent pathway.

Abstract

The current study was designed to evaluate the in vitro antiproliferative activity of 1,8-dihydroxy-4-methylanthracene-9,10-dione (DHMA) isolated from the Luffa acutangula against human non-small cell lung cancer cell line (NCI-H460). Induction of apoptosis and reactive oxygen species (ROS) generation was determined through fluorescence microscopic technique. Quantitative real-time PCR and western blotting analysis was carried out to detect the expression of pro-apoptotic (p53, p21, caspase-3, Bax, GADD45A, and ATM) and anti-apoptotic (NF-κB) proteins in NCI-H460 cell line. In silico studies also performed to predict the binding mechanism of DHMA with MDM2-p53 protein. The DHMA inhibited the cell viability of NCI-H460 cells in a dose-dependent manner with an IC(50) of about 50 µg/ml. It significantly reduced cell viability correlated with induction of apoptosis, which was associated with ROS generation. The apoptotic cell death was further confirmed through dual staining and DNA fragmentation assay. DHMA significantly increased the expression of anti-apoptotic protein such as p53, p21, Bax, and caspase-3 but downregulated the expression of NF-κB in NCI-H460 cell line. In silico studies demonstrate that DHMA formed hydrogen bond interaction with key residues Trp26, Phe55 and Lys24 by which it disrupt the binding of p53 with MDM2 receptor. These findings suggested that DHMA induces apoptosis in NCI-H460 via a p53-dependent pathway. This the first study on cytotoxic and apoptosis inducing activity of DHMA from L. acutangula against NCI-H460 cell line. Therefore, DHMA has therapeutic potential for lung cancer treatment.

• Authors: Ramar Vanajothi, Pappu Srinivasan
• Journal: Journal of receptor and signal transduction research (J Recept Signal Transduct Res) Vol. 36 Issue 3 Pg. 292-302 ( 2016) ISSN: 1532-4281 [Electronic] England
• PMID: 26585176 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthraquinones
• Cell Cycle Proteins
• Cyclin-Dependent Kinase Inhibitor p21
• Fluoresceins
• GADD45A protein, human
• NF-kappa B
• Nuclear Proteins
• RNA, Messenger
• Reactive Oxygen Species
• Tumor Suppressor Protein p53
• bcl-2-Associated X Protein
• 9,10-anthraquinone
• diacetyldichlorofluorescein
• Ataxia Telangiectasia Mutated Proteins
• Caspase 3
• Paclitaxel

Topics

• Anthraquinones (pharmacology)
• Apoptosis (drug effects, genetics)
• Ataxia Telangiectasia Mutated Proteins (genetics, metabolism)
• Carcinoma, Non-Small-Cell Lung (genetics, pathology)
• Caspase 3 (metabolism)
• Cell Cycle Proteins (genetics, metabolism)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cyclin-Dependent Kinase Inhibitor p21 (metabolism)
• DNA Fragmentation (drug effects)
• Fluoresceins (metabolism)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Intracellular Space (metabolism)
• Luffa (chemistry)
• Lung Neoplasms (genetics, metabolism, pathology)
• Models, Biological
• Molecular Docking Simulation
• Molecular Dynamics Simulation
• NF-kappa B (metabolism)
• Nuclear Proteins (genetics, metabolism)
• Paclitaxel (pharmacology)
• RNA, Messenger (genetics, metabolism)
• Reactive Oxygen Species (metabolism)
• Signal Transduction (drug effects)
• Tumor Suppressor Protein p53 (genetics, metabolism)
• bcl-2-Associated X Protein (genetics, metabolism)