Abstract | CONTEXT: OBJECTIVE: MATERIALS AND METHODS: For the acute toxicity study, the animals received orally five different single doses of β-D- mannuronic acid and were kept under observation for 14 d. In the subchronic study, 24 Wistar male rats were divided into four groups and were treated orally (gavage) once daily with test substance preparation at dose levels of 0, 50, 250, and 1250 mg/kg body weight for at least 63 consecutive days (9 weeks). Mortality, clinical signs, body weight changes, hematological and biochemical parameters, gross findings, organ weights, and histopathological determinations were monitored during the study. RESULTS: The results of acute toxicity indicated that the LD50 of β-D- mannuronic acid is 4.6 g/kg. We found no mortality and no abnormality in clinical signs, body weight, relative organ weights, or necropsy in any of the animals in the subchronic study. Additionally, the results showed no significant difference in hematological, biochemical, and histopathological parameters in rats. CONCLUSIONS: Our results suggest that β-D- mannuronic acid is relatively safe when administered orally in animals.
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Authors | Mohammad Javad Fattahi, Mohammad Abdollahi, Asghar Agha Mohammadi, Noushin Rastkari, Reza Khorasani, Hossein Ahmadi, Farzaneh Tofighi Zavareh, Reza Sedaghat, Nakisa Tabrizian, Abbas Mirshafiey |
Journal | Immunopharmacology and immunotoxicology
(Immunopharmacol Immunotoxicol)
Vol. 37
Issue 6
Pg. 535-40
( 2015)
ISSN: 1532-2513 [Electronic] England |
PMID | 26584020
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Hexuronic Acids
- mannuronic acid
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(toxicity)
- Dose-Response Relationship, Drug
- Drug Evaluation, Preclinical
(methods)
- Hexuronic Acids
(toxicity)
- Male
- Mice
- Rats
- Rats, Wistar
- Toxicity Tests, Acute
(methods)
- Toxicity Tests, Subchronic
(methods)
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