Longitudinal influence of microglial activation and amyloid on neuronal function in Alzheimer's disease.

Abstract

Amyloid deposition, tangle formation, neuroinflammation and neuronal dysfunction are pathological processes involved in Alzheimer's disease. However, the relative role of these processes in driving disease progression is still unclear. The aim of this positron emission tomography study was to: (i) investigate longitudinal changes of microglial activation, amyloid and glucose metabolism; and (ii) assess the temporospatial relationship between these three processes in Alzheimer's disease. A group of eight patients with a diagnosis of Alzheimer's disease (66 ± 4.8 years) and 14 healthy controls (65 ± 5.5 years) underwent T1 and T2 magnetic resonance imaging, along with (11)C-(R)-PK11195, (11)C-Pittsburgh compound B and (18)F-fluorodeoxyglucose positron emission tomography scans for microglial activation, amyloid deposition and glucose metabolism. All patients were followed-up with repeated magnetic resonance imaging and three positron emission tomography scans after 16 months. Parametric maps were interrogated using region of interest analysis, Statistical Parametric Mapping, and between-group correlation analysis at voxel-level using Biological Parametric Mapping. At baseline, patients with Alzheimer's disease showed significantly increased microglial activation compared to the control subjects. During follow-up, for the first time, we found that while there is a progressive reduction of glucose metabolism, there was a longitudinal increase of microglial activation in the majority of the patients with Alzheimer's disease. Voxel-wise correlation analysis revealed that microglial activation in patients with Alzheimer's disease was positively correlated with amyloid deposition and inversely correlated with regional cerebral metabolic rate at voxel level over time. Even though one of the limitations of this study is the lack of longitudinal follow-up of healthy control subjects, this study demonstrates that there is persistent neuroinflammation throughout the Alzheimer's disease process with associated synaptic dysfunction and reduced glucose metabolism. Voxel-wise correlation analysis suggests that neuroinflammation is associated with localized amyloid deposition and glucose metabolism over time, however, the level of inflammation could also occur independently of amyloid pathology, especially in the later stages of Alzheimer's disease.

• Authors: Zhen Fan, Aren A Okello, David J Brooks, Paul Edison
• Journal: Brain : a journal of neurology (Brain) Vol. 138 Issue Pt 12 Pg. 3685-98 (Dec 2015) ISSN: 1460-2156 [Electronic] England
• PMID: 26510952 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author (2015). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For Permissions, please email: [email protected].

Chemical References

• 2-(4'-(methylamino)phenyl)-6-hydroxybenzothiazole
• Amyloid
• Aniline Compounds
• Isoquinolines
• Thiazoles
• Fluorodeoxyglucose F18
• Glucose
• PK 11195

Topics

• Aged
• Alzheimer Disease (metabolism, pathology)
• Amyloid (metabolism)
• Aniline Compounds (metabolism)
• Brain (metabolism, pathology)
• Case-Control Studies
• Disease Progression
• Female
• Fluorodeoxyglucose F18 (metabolism)
• Follow-Up Studies
• Functional Neuroimaging
• Glucose (metabolism)
• Humans
• Isoquinolines (metabolism)
• Male
• Microglia (metabolism, pathology)
• Middle Aged
• Neurons (metabolism, pathology)
• Positron-Emission Tomography
• Thiazoles (metabolism)