Abstract |
MicroRNAs ( miRNAs) are known to be important post-transcription regulators of gene expression. Aberrant miRNA expression is associated with pathological disease processes, including carcinogenesis. Therefore, miRNAs are considered significant therapeutic targets for cancer therapy. MiRNAs do not act alone, but exhibit their functions by forming RNA-induced silencing complex (RISC). Thus, the regulation of RISC activity is a promising approach for cancer therapy. MiRNA is a core component of RISC and is an essential to RISC for recognizing target mRNA. Thereby, it is expected that development of the method to promote the release of miRNA from RISC would be an effective approach for inhibition of RISC activity. In this study, we synthesized novel peptide-conjugated oligonucleotides (RINDA-as) to promote the release of miRNA from RISC. RINDA-as showed a high rate of miRNA release from RISC and high level of inhibitory effect on RISC activity.
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Authors | Jumpei Ariyoshi, Daiki Momokawa, Nao Eimori, Akio Kobori, Akira Murakami, Asako Yamayoshi |
Journal | Bioconjugate chemistry
(Bioconjug Chem)
Vol. 26
Issue 12
Pg. 2454-60
(Dec 16 2015)
ISSN: 1520-4812 [Electronic] United States |
PMID | 26471458
(Publication Type: Journal Article)
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Chemical References |
- MicroRNAs
- Oligonucleotides, Antisense
- Peptides
- RNA-Induced Silencing Complex
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Topics |
- Base Sequence
- HEK293 Cells
- HeLa Cells
- Humans
- MicroRNAs
(metabolism)
- Oligonucleotides, Antisense
(chemistry, pharmacology)
- Peptides
(chemistry, pharmacology)
- RNA-Induced Silencing Complex
(antagonists & inhibitors, metabolism)
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