Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Abstract

Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

• Authors: Jingge Zhao, Zhaoqin Zhu, Xiaoyan Zhang, Yasuhiko Suzuki, Haorile Chagan-Yasutan, Haili Chen, Yanmin Wan, Jianqing Xu, Yugo Ashino, Toshio Hattori
• Journal: Journal of immunology research (J Immunol Res) Vol. 2015 Pg. 834749 ( 2015) ISSN: 2314-7156 [Electronic] Egypt
• PMID: 26339661 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Glycolipids
• Immunoglobulin A
• Immunoglobulin G
• Lipopolysaccharides
• lipoarabinomannan

Topics

• Adult
• Aged
• Antibodies, Bacterial (blood, immunology)
• Antigens, Bacterial (immunology)
• Case-Control Studies
• China (epidemiology)
• Comorbidity
• Enzyme-Linked Immunosorbent Assay
• Female
• Glycolipids (immunology)
• Humans
• Immunoglobulin A (blood, immunology)
• Immunoglobulin G (blood, immunology)
• Lipopolysaccharides (immunology)
• Male
• Middle Aged
• Mycobacterium tuberculosis (immunology)
• ROC Curve
• Tuberculosis (blood, diagnosis, epidemiology, immunology)