Protective effect of polyphenols in an inflammatory process associated with experimental pulmonary fibrosis in mice.

Abstract

Polyphenols have been described to have a wide range of biological activities, and many reports, published during recent years, have highlighted the beneficial effects of phenolic compounds, illustrating their promising role as therapeutic tools in several acute and chronic disorders. The purpose of study was to evaluate, in an already-assessed model of lung injury caused by bleomycin (BLM) administration, the role of resveratrol and quercetin, as well as to explore the potential beneficial properties of a mango leaf extract, rich in mangiferin, and a grape leaf extract, rich in dihydroquercetin (DHQ), on the same model. Mice were subjected to intra-tracheal administration of BLM, and polyphenols were administered by oral route immediately after BLM instillation and daily for 7 d. Treatment with resveratrol, mangiferin, quercetin and DHQ inhibited oedema formation and body weight loss, as well as ameliorated polymorphonuclear infiltration into the lung tissue and reduced the number of inflammatory cells in bronchoalveolar lavage fluid. Moreover, polyphenols suppressed inducible nitric oxide synthase expression, and prevented oxidative and nitroxidative lung injury, as shown by the reduced nitrotyrosine and poly (ADP-ribose) polymerase levels. The degree of apoptosis, as evaluated by Bid and Bcl-2 balance, was also suppressed after polyphenol treatment. Finally, these natural products down-regulated cyclo-oxygenase-2, extracellular signal-regulated kinase phosphorylated expression and reduced NF-κBp65 translocation. Our findings confirmed the anti-inflammatory effects of resveratrol and quercetin in BLM-induced lung damage, and highlight, for the first time, the protective properties of exogenous administration of mangiferin and DHQ on experimental pulmonary fibrosis.

• Authors: Daniela Impellizzeri, Elena Talero, Rosalba Siracusa, Antonio Alcaide, Marika Cordaro, Jose Maria Zubelia, Giuseppe Bruschetta, Rosalia Crupi, Emanuela Esposito, Salvatore Cuzzocrea, Virginia Motilva
• Journal: The British journal of nutrition (Br J Nutr) Vol. 114 Issue 6 Pg. 853-65 (Sep 28 2015) ISSN: 1475-2662 [Electronic] England
• PMID: 26334388 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Antioxidants
• Plant Extracts
• Polyphenols
• Stilbenes
• Xanthones
• mangiferin
• Quercetin
• taxifolin
• Resveratrol

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (analysis, therapeutic use)
• Antioxidants (analysis, therapeutic use)
• Apoptosis
• Dietary Supplements
• Disease Models, Animal
• Lung (immunology, metabolism, pathology)
• Male
• Mangifera (chemistry)
• Mice, Inbred ICR
• Neutrophil Infiltration
• Plant Extracts (chemistry, therapeutic use)
• Plant Leaves (chemistry)
• Polyphenols (analysis, therapeutic use)
• Pulmonary Fibrosis (immunology, metabolism, pathology, prevention & control)
• Quercetin (analogs & derivatives, analysis, therapeutic use)
• Random Allocation
• Respiratory Mucosa (immunology, metabolism, pathology)
• Resveratrol
• Stilbenes (therapeutic use)
• Vitis (chemistry)
• Xanthones (analysis, therapeutic use)