Correlation of survivin, p53 and Ki-67 in laryngeal cancer Hep-2 cell proliferation and invasion.

Abstract

OBJECTIVE:

To investigate the mechanism of survivin, p53 and Ki-67 on Hep-2 human laryngeal cancer endothelial cell proliferation and invasion.

METHODS:

Laryngeal squamous cell carcinoma and paracancerous normal tissues were collected, total RNA was extracted from tissues, survivin, p53 and Ki-67 gene mRNA expression levels in laryngeal cancer and the adjacent tissues were detected by Real-time PCR. Human laryngeal cancer Hep-2 epithelial cells were selected, survivin gene was overexpressed, and cell proliferation was detected by MTT. p53 and Ki-67 gene expression changes in overexpressed survivin gene were detected by Western blot. Changes in Hep-2 cell invasive ability were studied when survivin was overexpressed as detected by Transwell invasion assay.

RESULTS:

In the adjacent tissues, survivin, p53 and Ki-67 gene relative expression levels were 1.72 ± 0.9, 13.7 ± 5.7 and 5.7 ± 1.3, respectively; while in cancer tissues, gene relative expression levels were 53.7 ± 8.3, 66.7 ± 5.2 and 61.0 ± 3.1, respectively, which was significantly increased. As detected by MTT, relative cell survival rate within 12 h of survivin overexpression were: load control group (88.5 ± 1.6)%; overexpressed group (90.3 ± 1.9)%. Transwell invasion assay results indicated that overexpressed survivin could significantly increase the relative survival rate of cells.

CONCLUSIONS:

Expressions of p53, Ki67 and survivin are increased in cancer; and there is a positive correlation between survivin, p53 and Ki67 expressions in laryngeal carcinoma.

• Authors: Shi-Geng Pei, Ju-Xiang Wang, Xue-Ling Wang, Qing-Jun Zhang, Hong Zhang
• Journal: Asian Pacific journal of tropical medicine (Asian Pac J Trop Med) Vol. 8 Issue 8 Pg. 636-42 (Aug 2015) ISSN: 2352-4146 [Electronic] India
• PMID: 26321517 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.