Studying the relationship between cell cycle and Alzheimer's disease by gold nanoparticle probes.

Abstract

In this study, a simple gold nanoparticle (GNP)-based colorimetric assay has been developed for studying the relationship between cell cycle and β-amyloid peptide (Aβ, the biomarker of Alzheimer's disease [AD]) expression level. It was found that Aβ expression of neuronal cells (e.g., SHG-44 cell line) is strongly dependent on cell cycle phases; that is, the Aβ expression level was highest when cells were arrested in the G1/S phase by thymidine and was lowest when they were arrested in the G2/M phase by nocodazole. This finding may improve the understanding of AD pathology and provide a new tool for anti-dementia drug development.

• Authors: Chengke Wang, Zhenxin Wang
• Journal: Analytical biochemistry (Anal Biochem) Vol. 489 Pg. 32-7 (Nov 15 2015) ISSN: 1096-0309 [Electronic] United States
• PMID: 26299647 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Antimetabolites
• Biomarkers
• Immobilized Proteins
• Molecular Probes
• Nerve Tissue Proteins
• Peptide Fragments
• Tubulin Modulators
• amyloid beta-protein (1-16)
• Gold
• Streptavidin

Topics

• Alzheimer Disease (diagnosis, metabolism, pathology)
• Amyloid beta-Peptides (analysis, metabolism)
• Antimetabolites (pharmacology)
• Biomarkers (analysis, metabolism)
• Biotinylation
• Cell Line, Tumor
• Down-Regulation (drug effects)
• G1 Phase (drug effects)
• G2 Phase (drug effects)
• Gold (analysis, chemistry)
• Humans
• Immobilized Proteins (chemistry, metabolism)
• Metal Nanoparticles (analysis, chemistry)
• Molecular Probes (analysis, chemistry, metabolism)
• Nerve Tissue Proteins (analysis, metabolism)
• Neurons (chemistry, cytology, drug effects, pathology)
• Peptide Fragments (analysis, metabolism)
• Streptavidin (chemistry, metabolism)
• Surface Properties
• Tubulin Modulators (pharmacology)
• Up-Regulation (drug effects)