Moderate hypothermia ameliorates enterocyte mitochondrial dysfunction in severe shock and reperfusion.

Abstract

BACKGROUND:

Hypothermia can ameliorate ischemia-reperfusion-induced intestinal injury; however, whether the therapeutic mechanism of hypothermia on hemorrhagic shock, a severe condition of ischemia-reperfusion, is associated with mitochondrial protection in enterocytes is rarely reported. We aimed to evaluate the effects of hypothermia on mitochondria after shock-induced intestinal injury.

MATERIALS AND METHODS:

A severe hemorrhagic shock model was constructed in Sprague-Dawley rats at induced hypothermic (32°C or 34°C) or normothermic temperatures (37°C), followed by resuscitation with whole shed blood and Ringer lactate (15 mg/kg body weight). After 2 h, 24 rats were killed and their intestinal tissue was collected; the remaining animals were returned to the normothermic environment to observe the survival time.

RESULTS:

There was severe mitochondrial dysfunction in the normothermia group, as well as increased oxidative stress and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling apoptotic index. As expected, hypothermia treatment decreased mitochondrial permeability transition pore opening and restored the mitochondrial membrane potential and intracellular adenosine triphosphate content. Furthermore, hypothermia elevated mitochondrial-reduced glutathione and decreased mitochondrial malondialdehyde; consistent with the restored mitochondrial function, intestinal cell apoptosis and intestinal histopathologic injury were attenuated, the systemic inflammatory response was mitigated, and survival time was significantly prolonged. Additionally, moderate-induced hypothermia (32°C) had better therapeutic effects than mild hypothermia (34°C).

CONCLUSIONS:

The results suggest that moderate hypothermia resuscitation is an effective treatment for shock-induced intestinal injury, and its therapeutic mechanism may be related to mitochondrial protection.

• Authors: Wei Xiong, Shiyuan Xu, Hongyue Li, Kunhui Liang
• Journal: The Journal of surgical research (J Surg Res) Vol. 200 Issue 1 Pg. 250-9 (Jan 2016) ISSN: 1095-8673 [Electronic] United States
• PMID: 26227675 (Publication Type: Evaluation Study, Journal Article)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers

Topics

• Animals
• Apoptosis
• Biomarkers (metabolism)
• Blotting, Western
• Combined Modality Therapy
• Enterocytes (physiology)
• Fluid Therapy
• Hypothermia, Induced (methods)
• In Situ Nick-End Labeling
• Male
• Mitochondria (physiology)
• Oxidative Stress
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (etiology, prevention & control)
• Resuscitation
• Severity of Illness Index
• Shock, Hemorrhagic (physiopathology, therapy)