Even with significant advances in operative skills and adjuvant
therapies, the overall survival of patients suffering with head and neck squamous
cancers (
HNSCC) is unsatisfactory. Accordingly, no clinically useful prognostic
biomarkers have been found yet for
HNSCC. Many studies analysed the expression of potential markers in tumour tissues compared to adjacent tissues. Nevertheless, due to the sharing of the same microenvironment, adjacent tissues show molecular similarity to tumour tissues. Thus, gene expression patterns of 94
HNSCC tumorous tissues were compared with 31 adjacent tissues and with 10
tonsillectomy specimens of non-
cancer individuals. The genes analysed at
RNA level using quantitative RT-PCR and correlated with clinico-pathological conditions were as follows:
EGF, EGFR, MKI67, BCL2, BAX, FOS, JUN, TP53,
VEGF, FLT1, MMP2, MMP9, MT1A and MT2A. The elevated MT2A, BAX,
EGF and JUN expression was associated with the influence of tumour cells on the rearrangement of healthy tissues, as well as a significant shift in the BAX/BCL2 ratio. Our investigation also indicated that adjacent tissues play an important role in cancerogenesis by releasing several tumour-supporting factors such as
EGF. A gradual increase in the
metallothionein expression, from the lowest one in
tonsillectomy samples to the highest ones in tumour samples, suggests that MT expression might be tissue reaction to the presence of tumour cells. The results of this study confirmed the significance of
metallothionein in tumori-genesis and gave evidences for its use as a potential
HNSCC biomarker. Furthermore, this study highlighted the importance of histologically normal tumour-adjacent tissue in prediction of
HNSCC progress.