Abstract | PURPOSE: METHODS: In total, 1,052 urine, 150 serum, and 150 prostate tissue samples from patients with PCa or BPH were used in the study. A urine-based miRNA microarray analysis suggested the presence of differentially expressed urinary miRNAs in patients with PCa, and these were further validated in three independent PCa cohorts, using a quantitative reverse transcriptionpolymerase chain reaction analysis. RESULTS: The expression levels of hsa-miR-615-3p, hsv1-miR-H18, hsv2-miR-H9-5p, and hsa-miR-4316 were significantly higher in urine samples of patients with PCa than in those of BPH controls. In particular, herpes simplex virus (hsv)-derived hsv1-miR-H18 and hsv2-miR-H9-5p showed better diagnostic performance than did the serum prostate-specific antigen (PSA) test for patients in the PSA gray zone. Furthermore, a combination of urinary hsv2-miR-H9-5p with serum PSA showed high sensitivity and specificity, providing a potential clinical benefit by reducing unnecessary biopsies. CONCLUSIONS: Our findings showed that hsv-encoded hsv1-miR-H18 and hsv2-miR-H9-5p are significantly associated with PCa and can facilitate early diagnosis of PCa for patients within the serum PSA gray zone.
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Authors | Seok Joong Yun, Pildu Jeong, Ho Won Kang, Ye-Hwan Kim, Eun-Ah Kim, Chunri Yan, Young-Ki Choi, Dongho Kim, Jung Min Kim, Seon-Kyu Kim, Seon-Young Kim, Sang Tae Kim, Won Tae Kim, Ok-Jun Lee, Gou-Young Koh, Sung-Kwon Moon, Isaac Yi Kim, Jayoung Kim, Yung-Hyun Choi, Wun-Jae Kim |
Journal | International neurourology journal
(Int Neurourol J)
Vol. 19
Issue 2
Pg. 74-84
(Jun 2015)
ISSN: 2093-4777 [Print] Korea (South) |
PMID | 26126436
(Publication Type: Journal Article)
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