Functional characterization and expression analysis of myoglobin in high-altitude lizard Phrynocephalus erythrurus.

Abstract

Myoglobin (Mb) is a monomeric hemoprotein which plays an important role in oxygen storage and transport in cardiac and skeletal muscle under hypoxia. The red tail toad-headed lizard Phrynocephalus erythrurus (Lacertilia: Agamidae), which inhabits at an elevation of 4500-5300 m on the Qinghai-Tibetan plateau, is known to be the highest living lizard in the world. To investigate the characters of myoglobin of this unique species, another low altitude lizard Phrynocephalus przewalskii (Lacertilia: Agamidae) was selected as a reference. The open reading frame (ORF) of myoglobin in two lizards was 465 bp which encodes a polypeptide of 154 amino acids with different theoretical molecular weight and isoelectric point. The amino acid substitutions of myoglobin between two species were found at Thr13Ile, Lys87Thr and His118Asn. Homology modeling results indicated that P. erythrurus myoglobin has a greater heme pocket, which may be more favorable to oxygen binding and unloading. On the other hand, the mRNA levels of myoglobin in both cardiac and skeletal muscle in P. erythrurus were significantly larger than those in low altitude P. przewalskii. At protein level, myoglobin concentration in skeletal muscle in P. erythrurus was notably increased, but no significant difference was observed in cardiac muscle.

• Authors: Ying Xin, Xiaolong Tang, Huihui Wang, Songsong Lu, Yan Wang, Yang Zhang, Qiang Chen
• Journal: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology (Comp Biochem Physiol B Biochem Mol Biol) Vol. 188 Pg. 31-6 (Oct 2015) ISSN: 1879-1107 [Electronic] England
• PMID: 26116966 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Myoglobin
• RNA, Messenger
• Reptilian Proteins

Topics

• Acclimatization
• Altitude
• Animals
• Lizards (genetics, metabolism)
• Muscle, Skeletal (metabolism)
• Myocardium (metabolism)
• Myoglobin (chemistry, genetics, metabolism)
• RNA, Messenger (metabolism)
• Reptilian Proteins (chemistry, genetics, metabolism)