High expression of ErbB3 binding protein 1 (EBP1) predicts poor prognosis of pancreatic ductal adenocarcinoma (PDAC).

Abstract

Recent studies have identified that ErbB3 binding protein 1 (EBP1) is broadly expressed in various cancer tissues and critically involved in plenty of biological processes in this regard. However, the functional role of EBP1 in pancreatic ductal adenocarcinoma (PDAC) has never been elucidated. In this study, we found that EBP1 could serve as a prognostic biomarker of PDAC. Western blot analysis revealed that EBP1 was remarkably upregulated in PDAC tissues and cell lines. Using immunohistochemical analysis, we showed that the expression of EBP1 was correlated with tumor size (P = 0.004), histological differentiation (P = 0.041), and tumor node metastasis (TNM) stage (P = 0.000). Notably, Kaplan-Meier curve showed that high expression of EBP1 predicted significantly worsened prognosis of PDAC patients (P = 0.001). In addition, knockdown of EBP1 expression suppressed PDAC cell proliferation and retarded cell cycle progression. Furthermore, depletion of EBP1 induced the apoptosis of Panc-1 cells. Of great interest, we found that EBP1 interacted with anti-apoptotic protein, Bcl-xL, and promoted its accumulation. In summary, our results suggest that EBP1 is a novel prognostic indicator and potential therapeutic target of PDAC, shedding new insights into the important role of EBP1 in cancer development.

• Authors: Chen Gong, Yixin Zhang, Yinji Chen, Haifeng Zhang, Xiaorong Liu, Huaqing Xue, Li Ji, Liang Wang, Linlin Yang, Guoxiong Zhou, Chunhua Wan
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 36 Issue 12 Pg. 9189-99 (Dec 2015) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 26088450 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)

Chemical References

• Adaptor Proteins, Signal Transducing
• Biomarkers, Tumor
• PA2G4 protein, human
• RNA-Binding Proteins

Topics

• Adaptor Proteins, Signal Transducing (biosynthesis, genetics)
• Adenocarcinoma (genetics, pathology)
• Adult
• Aged
• Aged, 80 and over
• Apoptosis
• Biomarkers, Tumor (biosynthesis, genetics)
• Carcinoma, Pancreatic Ductal (genetics, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Kaplan-Meier Estimate
• Lymphatic Metastasis
• Male
• Middle Aged
• Neoplasm Staging
• Prognosis
• RNA-Binding Proteins (biosynthesis, genetics)