Simultaneous increase in multiple proinflammatory cytokines in the aqueous humor in neovascular glaucoma with and without intravitreal bevacizumab injection.

Abstract

PURPOSE:

To investigate aqueous humor proinflammatory cytokine levels of patients with neovascular glaucoma (NVG), and to analyze the effects of background factors in the expression of these molecules.

METHODS:

This cross-sectional study enrolled 137 participants who were grouped into (1) primary open-angle glaucoma (POAG; n = 36) patients; (2) NVG patients (NVG; n = 33); and (3) cataract surgery patients as a comparative group (CG; n = 68). Aqueous humor samples were collected from the anterior chamber at the start of surgery, deposited in CryoTubes, registered, and stored at -80 °C until processing. Multiplex microparticle-based immunodetection was performed by using xMAP and the Human Cytokine/Chemokine Panel I. Bevacizumab was injected into the vitreous cavity 1 to 2 days before surgery in 22 NVG patients (IVB group), whereas 11 NVG patients received no antivascular endothelial growth factor (VEGF) therapy 3 months preoperatively (N group). The Wilcoxon rank sum test or Fisher's exact test for two variables and the Tukey-Kramer honestly significant difference test for multiple variables were used to compare the cytokine levels.

RESULTS:

The NVG patients had higher levels of interleukin (IL)-6, IL-8, monocyte chemotactic protein (MCP)-1, tumor necrosis factor-α (TNF-α), and platelet-derived growth factor (PDGF)-AA compared to both the CG and POAG groups. The levels of IL-6, IL-8, MCP-1, and PDGF-AB/BB were higher in the IVB group than the N group, whereas the VEGF level was significantly lower in the IVB group (P < 0.01).

CONCLUSIONS:

Intravitreal bevacizumab injection decreased VEGF levels, but not those of the other cytokines.

• Authors: Saori Ohira, Toshihiro Inoue, Kohei Shobayashi, Keiichiro Iwao, Mikiko Fukushima, Hidenobu Tanihara
• Journal: Investigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 56 Issue 6 Pg. 3541-8 (Jun 2015) ISSN: 1552-5783 [Electronic] United States
• PMID: 26030108 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenesis Inhibitors
• Antibodies, Monoclonal, Humanized
• Cytokines
• Bevacizumab

Topics

• Adult
• Aged
• Aged, 80 and over
• Angiogenesis Inhibitors (administration & dosage)
• Animals
• Antibodies, Monoclonal, Humanized (administration & dosage)
• Aqueous Humor (metabolism)
• Bevacizumab
• Case-Control Studies
• Cataract (metabolism)
• Cross-Sectional Studies
• Cytokines (metabolism)
• Disease Models, Animal
• Female
• Glaucoma, Neovascular (drug therapy, metabolism)
• Glaucoma, Open-Angle (drug therapy, metabolism)
• Humans
• Intravitreal Injections
• Male
• Middle Aged
• Rabbits