Abstract | BACKGROUND: MATERIALS AND METHODS: The cells were incubated with physiological concentrations of [3H]- testosterone (5 nmol/L) alone or in the presence of either testosterone or androstenedione (0.5 and 50 μmol/L) 24 h at 37°C. Cellular radioactivity uptake was determined. [3H]-E2 was characterized by thin-layer chromatography. RESULTS: The MCF-7aro cells have a very high aromatase activity because conversion of [3H]- testosterone to [3H]-E2 was 3.02±0.17 pmol/mg DNA in non-treated cells. Testosterone, at concentrations of 0.5 and 50 μmol/L, provoked inhibition of E2 formation of 36% and 79%, respectively. The effect of androstenedione at 0.5 and 50 μmol/L was 56% and 76%, respectively. CONCLUSION:
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Authors | Gérard S Chetrite, Jorge R Pasqualini |
Journal | Hormone molecular biology and clinical investigation
(Horm Mol Biol Clin Investig)
Vol. 1
Issue 3
Pg. 147-53
(Jan 01 2010)
ISSN: 1868-1883 [Print] Germany |
PMID | 25961978
(Publication Type: Journal Article)
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