Changes in the intracellular calcium ion concentration in the gastric mucosa in a rat ischemia-reperfusion model.

Abstract

To investigate the role of the intracellular calcium ion in the development of acute gastric mucosal lesions, phosphorylase a activity was measured as an index of the intracellular calcium ion concentration ([Ca2+]i), using Lowry's method, in the rat ischemia-reperfusion model. [Ca2+]i increased significantly at the end of the ischemic state without acute mucosal lesions (AGML). After reinfusion, [Ca2+]i showed a slight increase and AGML developed. Continual intravenous infusion of the calcium channel blocker, diltiazem (1 mg/kg/hr), inhibited the increase in [Ca2+]i in the ischemic state and reduced the development of AGML after reinfusion. These results suggest that the increase in [Ca2+]i in the ischemic state plays an important role in the development of AGML.

• Authors: N Hayashi, M Tsujii, T Itoh, H Sakura, S Tsuji, H Tanimura, T Ogihara, H Yoshihara, S Kawano, N Sato
• Journal: Scandinavian journal of gastroenterology. Supplement (Scand J Gastroenterol Suppl) Vol. 162 Pg. 43-6 ( 1989) ISSN: 0085-5928 [Print] England
• PMID: 2595305 (Publication Type: Journal Article)

Chemical References

• Ions
• Phosphorylase a
• Diltiazem
• Calcium

Topics

• Animals
• Calcium (metabolism)
• Diltiazem (pharmacology)
• Gastric Mucosa (blood supply, metabolism, ultrastructure)
• Intracellular Membranes (metabolism)
• Ions
• Male
• Osmolar Concentration
• Phosphorylase a (metabolism)
• Rats
• Rats, Inbred Strains
• Reperfusion Injury (metabolism)