Evaluation of anti-osteoarthritic activity of Vigna mungo in papain induced osteoarthritis model.

Abstract

AIM:

This study was carried out to evaluate the effect of Vigna mungo hydroalcoholic extract (VMHA) by papain induced osteoarthritis (OA) in the rat model.

MATERIALS AND METHODS:

OA was induced by intra-articular injection of papain (4% w/v) along with cysteine (0.03 M) on day 1, 4 and 7 in rats and VMHA was administered orally in three doses (100, 200 and 400 mg/kg) after last papain injection. The anti-osteoarthritic activity was evaluated by measuring knee joint diameter, grip strength, locomotion activity and hanging time. Histopathological analysis and acute toxicity study were also performed.

RESULTS:

VMHA improved inflammatory condition with all the doses, but significant (P < 0.05) attenuation of inflammation was present only with 400 mg/kg dose. The grip strength, locomotion activity and hanging time were also significantly (P < 0.05) improved at dose level of 100 mg/kg however other two doses (200 mg/kg and 400 mg/kg) were not found to be effective. VMHA did not show any mortality or any toxic clinical signs after oral administration of 2 g/kg dose.

CONCLUSION:

VMHA improved arthritic condition by significantly reducing pain and inflammation.

• Authors: Dhaval V Patel, Mrunal Ghag Sawant, Ginpreet Kaur
• Journal: Indian journal of pharmacology (Indian J Pharmacol) 2015 Jan-Feb Vol. 47 Issue 1 Pg. 59-64 ISSN: 1998-3751 [Electronic] India
• PMID: 25821313 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Analgesics
• Anti-Inflammatory Agents
• Plant Extracts
• Papain
• Celecoxib

Topics

• Administration, Oral
• Analgesics (administration & dosage, pharmacology, toxicity)
• Animals
• Anti-Inflammatory Agents (administration & dosage, pharmacology, toxicity)
• Arthralgia (drug therapy, physiopathology)
• Celecoxib (pharmacology)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Fabaceae
• Female
• Hand Strength
• Knee Joint (drug effects, pathology, physiopathology)
• Motor Activity (drug effects)
• Osteoarthritis, Knee (chemically induced, drug therapy, pathology, physiopathology)
• Papain
• Phytotherapy
• Plant Extracts (administration & dosage, pharmacology, toxicity)
• Plants, Medicinal
• Rats, Wistar
• Seeds