Ameliorative effects of Gualou Guizhi decoction on inflammation in focal cerebral ischemic-reperfusion injury.

Abstract

Gualou Guizhi decoction (GLGZD) is a well-established Traditional Chinese Medicinal formulation which has long been used to treat stroke in a clinical setting in China. The present study investigated the ameliorative effects of GLGZD on inflammation in focal cerebral ischemic-reperfusion injury. A rat model of middle cerebral artery occlusion (MCAO) was employed. Rats were administrated GLGZD (7.2 and 14.4 g/kg per day) or saline as control 2 h after reperfusion and daily over the following seven days. Neurological deficit score and screen test were evaluated at 1, 3, 5 and 7 days after MCAO. Brain infarct size and brain histological changes were observed via 2,3,5-triphenyltetrazolium chloride staining and regular hematoxylin & eosin staining. Furthermore, inflammation mediators and nuclear factor-κB (NF-κB) were investigated using ELISA and immunohistochemistry. GLGZD treatment significantly improved neurological function, ameliorated histological changes to the brain and decreased infarct size in focal cerebral ischemic-reperfusion injury. GLGZD was found to significantly reduce interleukin (IL)-1, tumor necrosis factor-α and NF-κB levels, while increasing levels of IL-10. In conclusion, the present study suggested that GLGZD has a neuroprotective effect on focal cerebral ischemic-reperfusion injury and this effect is likely to be associated with the anti-inflammatory function of GLGZD.

• Authors: Yuqin Zhang, Shengnan Zhang, Huang Li, Mei Huang, Wei Xu, Kedan Chu, Lidian Chen, Xianwen Chen
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 12 Issue 1 Pg. 988-94 (Jul 2015) ISSN: 1791-3004 [Electronic] Greece
• PMID: 25815894 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Drugs, Chinese Herbal
• Interleukin-1
• NF-kappa B
• Neuroprotective Agents
• Tumor Necrosis Factor-alpha
• guizhi decoction
• Interleukin-10

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Brain Ischemia (drug therapy, genetics, immunology, pathology)
• Disease Models, Animal
• Drugs, Chinese Herbal (pharmacology)
• Gene Expression
• Histocytochemistry
• Infarction, Middle Cerebral Artery (drug therapy, genetics, immunology, pathology)
• Inflammation (drug therapy, genetics, immunology, pathology)
• Interleukin-1 (antagonists & inhibitors, genetics, immunology)
• Interleukin-10 (agonists, genetics, immunology)
• Male
• NF-kappa B (antagonists & inhibitors, genetics, immunology)
• Neuroprotective Agents (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy, genetics, immunology, pathology)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors, genetics, immunology)