[Comparative study of HR HPV E6/E7 mRNA and HR-HPV DNA in cervical cancer screening].

Abstract

OBJECTIVE:

To determine the performance of HR-HPV E6/E7 massager RNA (mRNA) test for detecting high-grade cervical intraepithelial neoplasia in cervical cancer screening and compare the clinical performance of HR-HPV E6/E7 mRNA test with HC-2 and Cervista HPV DNA tests for cross-sectional positivity in women with and without cervical neoplasia.

METHODS:

A total of 172 women underwent cytology, HR-HPV DNA test, HR-HPV E6/7 mRNA test, colposcopy and biopsy. We compared the clinical performance of HR-HPV E6/E7 mRNA test with Hybrid Capture 2 DNA test (HC-2) and Cervista HR-HPV DNA test on the cervical brush specimens during colposcopy and routine screening. The samples were histologically confirmed high-grade cervical intraepithelial neoplasia (CIN II) or worse (CIN II+) as an endpoint.

RESULTS:

HR-HPV E6/E7 mRNA positive rate was 37.9% in NILM, 67.9% in ASCUS and LSIL, 88.5% in ASC-H+. HR-HPV E6/E7 mRNA positive rate was 38.6% in CIN I, 77.4% in CIN II-3 and 92.5% in SCC. HR-HPV E6/E7 mRNA test showed a higher specificity than HC-2 and Cervista HPV DNA tests for high-grade lesions (61.4%, 54.3%, 55.7%, respectively, P < 0.05) and also a higher positive predictive value (75.9%, 74.8%, 74.6% respectively). Among three tests, HR-HPV E6/E7 mRNA had the largest area of ROC curve and the best diagnostic value.

CONCLUSION:

HR-HPV E6/E7 mRNA test has a performance more specific for detecting CIN II+ with the same sensitivity as HC-2 and Cervista HPV DNA tests. And it may serve as a more specific test for predicting the risk of progression and offer a viable tool for triage during cervical cancer screening.

• Authors: Xuye Zhao, Yong Cui, Shufang Jiang, Yuanguang Meng, Aijun Liu, Linping Wei, Tongyu Liu, Haiqiong Han, Xiaosu Liu, Fuhong Liu, Yali Li
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 94 Issue 43 Pg. 3432-5 (Nov 25 2014) ISSN: 0376-2491 [Print] China
• PMID: 25622677 (Publication Type: Comparative Study, Journal Article)

Chemical References

• DNA, Viral
• Oncogene Proteins, Viral
• RNA, Messenger
• RNA, Viral

Topics

• Biopsy
• Colposcopy
• Cross-Sectional Studies
• DNA, Viral
• Early Detection of Cancer
• Female
• Humans
• Oncogene Proteins, Viral
• Papillomaviridae
• Pregnancy
• RNA, Messenger
• RNA, Viral
• Uterine Cervical Dysplasia
• Uterine Cervical Neoplasms