[CCL21 promotes the metastasis of human pancreatic cancer Panc-1 cells via epithelial- mesenchymal transition].

Abstract

OBJECTIVE:

To investigate the mechanism underlying that chemokine (C-C motif) ligand 21 (CCL21) promotes the metastasis ability of human pancreatic cancer Panc-1 cells.

METHODS:

Transwell(TM) was used to access the chemotaxis effect of CCL21 on Panc-1 cells. Real-time quantitative PCR was performed to detect the expression of C-C chemokine receptor type 7 (CCR7) mRNA in the upper and lower chambers. Immunofluorescence staining and Western blotting were employed to examine the expressions of the epithelial-mesenchymal transition (EMT)-related proteins and CD133 of Panc-1 cells in the lower chamber, which were compared with those of the upper chamber as the control.

RESULTS:

The numbers of the Panc-1 cells induced by 0, 50, 100, 200 ng/mL CCL21 were 13.00 ± 3.00, 78.00 ± 9.00, 161.00 ± 11.00, 281.00 ± 17.00, respectively; with the increase of the concentration of CCL21, there were more cells migrating from the upper to the lower chamber; and the cells in the lower chamber expressed higher level of CCR7 mRNA than the ones staying in the upper chamber. The relative protein expressions of MMP-9, vimentin, E-cadherin and CD133 in the lower chamber were 0.42 ± 0.04, 0.36 ± 0.03, 0.12 ± 0.02, 0.46 ± 0.03, respectively, which were statistically significantly different from those in the upper chamber (0.15 ± 0.02, 0.25 ± 0.02, 0.25 ± 0.03, 0.13 ± 0.02, respectively).

CONCLUSION:

CCL21/CCR7 axis maybe play an important role in the metastasis of pancreatic cancer stem cells by EMT and up-regulation of MMP-9.

• Authors: Qing Liu, Fangfang Chen, Tanghai Duan, Haitao Zhu, Xiaodong Xie, Yingying Wu, Zhijian Zhang, Dongqing Wang
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 31 Issue 1 Pg. 6-9, 13 (Jan 2015) ISSN: 1007-8738 [Print] China
• PMID: 25575049 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokine CCL21
• Vimentin
• Matrix Metalloproteinase 9

Topics

• Cell Line, Tumor
• Chemokine CCL21 (genetics, metabolism)
• Epithelial-Mesenchymal Transition
• Humans
• Matrix Metalloproteinase 9 (genetics, metabolism)
• Neoplasm Metastasis
• Pancreatic Neoplasms (genetics, metabolism, pathology, physiopathology)
• Vimentin (genetics, metabolism)