Abstract |
An ultra-performance liquid chromatography-quadrupole time-of-flight-based metabolomic approach was developed to study influence of salidroside, an anti- fatigue ingredient from Rhoiola rosea, on urinary metabolic profiling of rats to a single dose of 180 mg/kg per day. Unsupervised principal component analysis (PCA) and supervised orthogonal pre-projection to latent structures discriminate analysis (OPLS-DA) on metabolite profiling revealed obvious differentiation between the salidroside treated groups and controls in both positive and negative ion modes. Eleven urinary metabolites contributing to the differentiation were identified as anti- fatigue biomarkers: N-acetylserotonin, 2-Methoxyestrone 3-glucuronide, Taurine, Melatonin, Sorbitol, Geranyl diphosphate, Z-nucleotide, Cortisone, Dihydrocortisol, Sebacic acid, Pregnenolone sulfate. The physiological significance of these biomarkers is discussed. The work showed that metabolomics is a powerful tool in studying the anti- fatigue effects of natural compound salidroside on multiple targets in vivo.
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Authors | Chaoyang Ma, Liming Hu, Guanjun Tao, Wenping Lv, Hongxin Wang |
Journal | Journal of pharmaceutical and biomedical analysis
(J Pharm Biomed Anal)
Vol. 105
Pg. 84-90
(Feb 2015)
ISSN: 1873-264X [Electronic] England |
PMID | 25543286
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers
- Glucosides
- Phenols
- rhodioloside
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Topics |
- Animals
- Biomarkers
(urine)
- Chromatography, High Pressure Liquid
(instrumentation, methods)
- Fatigue
(drug therapy, urine)
- Glucosides
(isolation & purification, urine)
- Male
- Metabolomics
(instrumentation, methods)
- Mice, Inbred Strains
- Multivariate Analysis
- Phenols
(isolation & purification, urine)
- Principal Component Analysis
- Rats
- Rhodiola
(chemistry)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
(instrumentation, methods)
- Swimming
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