HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

An UPLC-MS-based metabolomics investigation on the anti-fatigue effect of salidroside in mice.

Abstract
An ultra-performance liquid chromatography-quadrupole time-of-flight-based metabolomic approach was developed to study influence of salidroside, an anti-fatigue ingredient from Rhoiola rosea, on urinary metabolic profiling of rats to a single dose of 180 mg/kg per day. Unsupervised principal component analysis (PCA) and supervised orthogonal pre-projection to latent structures discriminate analysis (OPLS-DA) on metabolite profiling revealed obvious differentiation between the salidroside treated groups and controls in both positive and negative ion modes. Eleven urinary metabolites contributing to the differentiation were identified as anti-fatigue biomarkers: N-acetylserotonin, 2-Methoxyestrone 3-glucuronide, Taurine, Melatonin, Sorbitol, Geranyl diphosphate, Z-nucleotide, Cortisone, Dihydrocortisol, Sebacic acid, Pregnenolone sulfate. The physiological significance of these biomarkers is discussed. The work showed that metabolomics is a powerful tool in studying the anti-fatigue effects of natural compound salidroside on multiple targets in vivo.
AuthorsChaoyang Ma, Liming Hu, Guanjun Tao, Wenping Lv, Hongxin Wang
JournalJournal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 105 Pg. 84-90 (Feb 2015) ISSN: 1873-264X [Electronic] England
PMID25543286 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier B.V. All rights reserved.
Chemical References
  • Biomarkers
  • Glucosides
  • Phenols
  • rhodioloside
Topics
  • Animals
  • Biomarkers (urine)
  • Chromatography, High Pressure Liquid (instrumentation, methods)
  • Fatigue (drug therapy, urine)
  • Glucosides (isolation & purification, urine)
  • Male
  • Metabolomics (instrumentation, methods)
  • Mice, Inbred Strains
  • Multivariate Analysis
  • Phenols (isolation & purification, urine)
  • Principal Component Analysis
  • Rats
  • Rhodiola (chemistry)
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (instrumentation, methods)
  • Swimming

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: