Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis.

Abstract

In this in-vitro study, the effect of prohibitin (PHB) on glucose metabolism in eutopic endometrial stromal cells from women with endometriosis was investigated. Endometrial stromal cells were isolated from endometrium in women with endometriosis, in women without endometriosis, or from endometrioma tissues. Glucose metabolic phenotype of stromal cells were examined in vitro. Quantitative polymerase chain reaction was used to measure the mRNA expression of glycolysis-related genes. Glucose consumption and lactate production were examined after knockdown of PHB expression in women with endometriosis with siRNA. In endometrioma tissue, significantly increased glucose consumption, lactate production and aberrant expression of glycolysis-related enzymes were found in women with endometriosis compared with women who do not have endometriosis (P < 0.05 versus P < 0.001). In women with endometriosis, PHB mRNA and protein were under-expressed in endometrioma tissue; in women without endometriosis, PHB mRNA and protein were over-expressed. Knockdown of PHB expression in women with endometriosis increased glucose consumption, although it had no effect on lactate production. This study suggests that aberrant expression of glycolysis-related enzymes in endometrioma tissue is associated with enhanced glycolytic metabolism. The malignant-like feature may be partially caused by low-expression of PHB gene in endometriotic stromal cells.

• Authors: Xiaochen Qi, Yanxin Zhang, Hui Ji, Xiadi Wu, Fuxin Wang, Manxin Xie, Li Shu, Shiwen Jiang, Yundong Mao, Yugui Cui, Jiayin Liu
• Journal: Reproductive biomedicine online (Reprod Biomed Online) Vol. 29 Issue 6 Pg. 761-70 (Dec 2014) ISSN: 1472-6491 [Electronic] Netherlands
• PMID: 25444511 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Chemical References

• PHB protein, human
• Prohibitins
• RNA, Small Interfering
• Repressor Proteins
• Lactic Acid
• Glucose

Topics

• Adult
• Endometriosis (metabolism)
• Endometrium (cytology)
• Female
• Gene Knockdown Techniques
• Glucose (metabolism)
• Humans
• In Vitro Techniques
• Lactic Acid
• Prohibitins
• RNA Interference
• RNA, Small Interfering (genetics)
• Real-Time Polymerase Chain Reaction
• Repressor Proteins (metabolism)
• Stromal Cells (metabolism)