Active hexose correlated compound exerts therapeutic effects in lymphocyte driven colitis.

Abstract

Active hexose correlated compound (AHCC) is a commercial extract of Basidiomycetes fungi enriched in oligosaccharides that is used as a human nutritional supplement for various purposes in humans. Our aim was to study the anti-inflammatory effect of AHCC in the CD4+ CD62L(+) T cell transfer model of colitis, considered one of the closest to the human disease. Colitis was induced by transfer of CD4(+) CD62L(+) T cells to recombination activating gene 1(-/-) mice. AHCC (75 mg/d) was administered by gavage as a post-treatment. Three groups were established: noncolitic, colitic (CD4(+) CD62L(+) transferred mice treated with vehicle), and AHCC (colitic treated with AHCC). AHCC improved colitis, as evidenced by a 24% lower colonic myeloperoxidase and a 21% lower alkaline phosphatase activity. In addition, a decreased secretion of proinflammatory genes assessed by RT-qPCR was observed, particularly TNF-α and IL-1β. Ex vivo mesenteric lymph node cells obtained from AHCC treated mice exhibited a fully normalized production of IL-6, IL-17, and IL-10 (p < 0.05). Also, AHCC treated mice exhibited decreased STAT4 and IκB-α phosphorylation in splenic CD4(+) cells. Our data provide validation of AHCC colonic anti-inflammatory activity in a chronic, T cell driven model of inflammatory bowel disease.

• Authors: Cristina Mascaraque, María Dolores Suárez, Antonio Zarzuelo, Fermín Sánchez de Medina, Olga Martínez-Augustin
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 58 Issue 12 Pg. 2379-82 (Dec 2014) ISSN: 1613-4133 [Electronic] Germany
• PMID: 25186628 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Anti-Inflammatory Agents
• I-kappa B Proteins
• Interleukin-17
• Interleukin-1beta
• Interleukin-6
• NFKBIA protein, human
• Nfkbia protein, mouse
• Polysaccharides
• STAT4 Transcription Factor
• Stat4 protein, mouse
• Tumor Necrosis Factor-alpha
• Interleukin-10
• NF-KappaB Inhibitor alpha
• Active Hexose Correlated Compound

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• CD4-Positive T-Lymphocytes (drug effects, metabolism)
• Colitis (drug therapy)
• Colon (drug effects, pathology)
• Disease Models, Animal
• I-kappa B Proteins (genetics, metabolism)
• Interleukin-10 (metabolism)
• Interleukin-17 (metabolism)
• Interleukin-1beta (genetics, metabolism)
• Interleukin-6 (metabolism)
• Mice
• NF-KappaB Inhibitor alpha
• Phosphorylation
• Polysaccharides (pharmacology)
• Reproducibility of Results
• STAT4 Transcription Factor (genetics, metabolism)
• Spleen (cytology, drug effects)
• Tumor Necrosis Factor-alpha (genetics, metabolism)