Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: In this study, TQ elicited a strong cytotoxic effect on SASVO3, a highly malignant HNSCC cell line. The mechanisms of this cytotoxic effect were concentration dependent. TQ also induced apoptotic cell death in SASVO3 cells as indicated by an increase in Bax expression and caspase-9 activation. Apoptosis was possibly caspase-9 dependent because the exposure of cells to a caspase-9 inhibitor partially prevented cell death. The exposed cells also showed increased levels of autophagic vacuoles and LC3-II proteins, which are specific autophagy markers. Cell viability assay results further revealed that bafilomycin-A1, an autophagy inhibitor, enhanced TQ cytotoxicity; by comparison, Annexin V and propidium-iodide staining assay results showed that this inhibitor did not promote apoptosis. TQ treatment also increased the accumulation of autophagosomes. Using a lentivirus- shRNA system for LC3 silencing, we found that cell viability was eradicated in autophagy-defective cells. An in vivo BALB/c nude mouse xenograft model further showed that TQ administered by oral gavage reduced tumor growth via induced autophagy and apoptosis. CONCLUSIONS: These findings indicated that TQ induced cell death in oral cancer cells via two distinct anti-neoplastic activities that can induce apoptosis and autophagy. Therefore, TQ is a promising candidate in phytochemical-based, mechanistic, and pathway-targeted cancer prevention strategies.
|
Authors | Shu-Chen Chu, Yih-Shou Hsieh, Cheng-Chia Yu, Yi-Yeh Lai, Pei-Ni Chen |
Journal | PloS one
(PLoS One)
Vol. 9
Issue 7
Pg. e101579
( 2014)
ISSN: 1932-6203 [Electronic] United States |
PMID | 25000169
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antineoplastic Agents
- Benzoquinones
- MAP1LC3A protein, human
- Macrolides
- Microtubule-Associated Proteins
- bafilomycin A1
- Caspases
- thymoquinone
|
Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Autophagy
(drug effects)
- Benzoquinones
(pharmacology)
- Carcinoma, Squamous Cell
(pathology)
- Caspases
(metabolism)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Drug Synergism
- Enzyme Activation
(drug effects)
- Head and Neck Neoplasms
(pathology)
- Humans
- Macrolides
(pharmacology)
- Mice
- Microtubule-Associated Proteins
(metabolism)
- Phagosomes
(drug effects)
- Squamous Cell Carcinoma of Head and Neck
- Xenograft Model Antitumor Assays
|