Tumor growth and
metastasis are responsible for most
cancer patients' deaths. Here, we report that eupolyphaga sinensis walker has an essential role in resisting
hepatocellular carcinoma growth and
metastasis. Compared with proliferation, colony formation, transwell assay and transplantable
tumor in nude mouse in vitro and vivo, eupolyphaga sinensis walker extract (ESWE) showed good inhibition on the SMMC-7721 cell growth and
metastasis. Using genome-wide microarray analysis, we found the down-regulated growth and
metastasis factors, and selected down-regulated genes were confirmed by real-time PCR. Knockdown of a checkpoint PKCβ by
siRNA significantly attenuated
tumor inhibition and
metastasis effects of ESWE. Moreover, our results indicate ESWE inhibits HCC growth by not only downregulating the signaling of PKCβ, Akt, m-TOR, Erk1/2, MEK-2, Raf and JNK-1, but also increasing
cyclin D1 protein levels and decreasing amount of
cyclin E,
cyclin B1 and cdc2 of the cycle
proteins. At the same time, ESWE reduced MMP2, MMP9 and CXCR4, PLG, NFκB and P53 activities. Overall, our studies demonstrate that ESWE is a key factor in growth and
metastasis signaling inhibitor targeting the PKC, AKT, MAPK signaling and related
metastasis signaling, having potential in
cancer therapy.