Abstract | PURPOSE: To compare dynamic 2-deoxy-2-[(18) F]fluoro- D-glucose positron emission tomography ((18) F-FDG PET) parameters in two selected human breast cancer xenografts and to evaluate associations with immunohistochemistry and histology. PROCEDURES: Dynamic (18) F-FDG PET of luminal-like MAS98.06 and basal-like MAS98.12 xenografts was performed, and the compartmental transfer rates (k 1 ,k 2 ,k 3 ), blood volume fraction (v B ) and metabolic rate of (18) F-FDG(MR FDG ) were estimated from pharmacokinetic model analysis. After sacrifice, analyses of hypoxia ( pimonidazole), proliferation (Ki-67), vascularization (CD31), glucose transport receptor (GLUT1) and necrosis (HE) was performed. The level of hexokinase 2 (HK2) was estimated from Western blot analysis. RESULTS: The (18) F-FDG uptake curves for the two xenografts were significantly different (p < 0.05). k 1 and v B were higher for MAS98.12 (p < 0.01), while k 3 was higher for MAS98.06 (p < 0.01). MAS98.12 had a higher fraction of stromal tissue and higher microvessel density (MVD), and it was less necrotic and hypoxic than MAS98.06. MAS98.12 had stronger positive GLUT1 staining and lower Ki-67 than MAS98.06. In both models significant correlations were found between k 1 and the GLUT1 score, between k 3 and the level of HK2, and between v B and MVD. CONCLUSIONS: Significant differences in dynamic (18) F-FDG parameters between the two human breast cancer xenografts were found. The differences could be explained by underlying histological and physiological characteristics.
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Authors | Alexandr Kristian, Line B Nilsen, Kathrine Røe, Mona-Elisabeth Revheim, Olav Engebråten, Gunhild M Mælandsmo, Ruth Holm, Eirik Malinen, Therese Seierstad |
Journal | Nuclear medicine and molecular imaging
(Nucl Med Mol Imaging)
Vol. 47
Issue 3
Pg. 173-80
(Sep 2013)
ISSN: 1869-3474 [Print] Germany |
PMID | 24900104
(Publication Type: Journal Article)
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