Oral administration of the milk casein-derived tripeptide Val-Pro-Pro attenuates high-fat diet-induced adipose tissue inflammation in mice.

Abstract

Inflammation of adipose tissue triggers the metabolic syndrome, atherosclerosis and CHD. In the present study, we investigated whether the milk casein-derived tripeptide valine-proline-proline (VPP) has an anti-inflammatory effect on the adipose tissue of high-fat diet (HFD)-fed mice. Male C57BL/6J mice (7 weeks of age) were fed ad libitum with either a HFD and plain tap water (HFD group) or a HFD and water containing 0·3 mg VPP/ml (HFD+VPP group) for 10 weeks. The results showed that the expression level of CD18 in the peripheral blood monocytes of the HFD+VPP group was significantly decreased compared with the level observed in those of the HFD group. Activated monocytes and pro-inflammatory macrophages were accumulated in the stromal vascular fractions of the adipose tissue from HFD-fed mice, which were significantly decreased in those supplemented with VPP. The formation of crown-like structures rich in pro-inflammatory macrophages was also significantly reduced in the adipose tissue of mice administered with VPP. Real-time PCR analysis revealed that the expression of monocyte chemoattractant protein-1 and that of the pro-inflammatory cytokine IL-6 in adipose tissue tend to be lower in the HFD+VPP group than in the HFD group. These observations indicate that oral administration of VPP exerts an anti-inflammatory effect on the adipose tissue of HFD-fed mice, which may eventually lead to the primary prevention of chronic inflammation-related diseases.

• Authors: Kotaro Aihara, Mizuko Osaka, Masayuki Yoshida
• Journal: The British journal of nutrition (Br J Nutr) Vol. 112 Issue 4 Pg. 513-9 (Aug 28 2014) ISSN: 1475-2662 [Electronic] England
• PMID: 24870967 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• CD18 Antigens
• Caseins
• Ccl2 protein, mouse
• Chemokine CCL2
• Interleukin-6
• Oligopeptides
• Peptide Fragments
• interleukin-6, mouse
• valyl-prolyl-proline

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (therapeutic use)
• CD18 Antigens (blood, metabolism)
• Caseins (chemistry, therapeutic use)
• Cattle
• Chemokine CCL2 (antagonists & inhibitors, genetics, metabolism)
• Diet, High-Fat (adverse effects)
• Dietary Supplements
• Gene Expression Regulation
• Interleukin-6 (antagonists & inhibitors, genetics, metabolism)
• Intra-Abdominal Fat (immunology, metabolism, pathology)
• Macrophages (immunology, metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Monocytes (immunology, metabolism)
• Obesity (diet therapy, immunology, metabolism, physiopathology)
• Oligopeptides (therapeutic use)
• Panniculitis (etiology, prevention & control)
• Peptide Fragments (therapeutic use)