Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis.

Abstract

LncRNA SPRY4-IT1 has been shown to promote the progression of melanoma. However, the role of lncRNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) remains unclear. The purpose of this study is to investigate the clinical significance and biological functions of SPRY4-IT1 in ESCC. The expression levels of lncRNA SPRY4-IT in 92 ESCC patients and 8 ESCC cell lines were evaluated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The prognostic significance was evaluated using Kaplan-Meier and Cox regression analyses. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in ESCC cell lines. Both in vitro and in vivo assays were performed to further explore its role in tumor progression. SPRY4-IT1 levels were significantly higher in ESCC tissues and cells than in corresponding adjacent noncancerous tissues and nontumorigenic esophageal epithelial cells, and the ESCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. The multivariate analysis revealed that SPRY4-IT1 expression level is an independent prognostic factor in ESCC patients. In vitro assays demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration. In vivo assays demonstrated that knockdown of SPRY4-IT1 decreases cell growth. SPRY4-IT1 is a novel molecule involved in ESCC progression, which may provide a potential prognostic biomarker and a potential target for therapeutic intervention.

• Authors: Hai-Wei Xie, Qing-Quan Wu, Bin Zhu, Fang-Jun Chen, Lv Ji, Su-Qing Li, Chun-Mei Wang, Yu-Suo Tong, Lei Tuo, Ming Wu, Zhi-Hua Liu, Jin Lv, Wei-Hong Shi, Xiu-Feng Cao
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 35 Issue 8 Pg. 7743-54 (Aug 2014) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 24810925 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intracellular Signaling Peptides and Proteins
• Nerve Tissue Proteins
• RNA, Long Noncoding
• SPRY4 protein, human

Topics

• Aged
• Animals
• Carcinoma, Squamous Cell (genetics, mortality)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Esophageal Neoplasms (genetics, mortality)
• Esophageal Squamous Cell Carcinoma
• Female
• Humans
• Intracellular Signaling Peptides and Proteins (genetics)
• Male
• Mice
• Mice, Inbred BALB C
• Middle Aged
• Neoplasm Invasiveness
• Nerve Tissue Proteins (genetics)
• Prognosis
• RNA, Long Noncoding (genetics, physiology)
• Up-Regulation