Abstract |
Th9 cells have been reported to contribute to immune responses; however, the role of Th9 cells in Echinococcus granulosus infection is unknown. This study is to determine whether Th9 cells and IL-9 are involved in human Echinococcus granulosus infection. Compared with healthy controls (HC group), the mRNA levels of PU.1, IL-9, and GATA-3 were significantly increased in patients before therapy (CE group), as revealed by qRT-PCR. Flow cytometry analysis showed that the percentages of Th9 and Th2 cells in CE group were significantly higher. The levels of IL-9, IL-4, IL-10, and TGF- β in CE group were also significantly increased, as detected by CBA assay. The percentages of Th9 and Th2 cells in CE group were positively correlated. After treatments of surgery in combination with albendazole, the PU.1 and GATA-3 mRNA levels were significantly decreased in patients after therapy (PCE group) compared with CE group. The numbers of Th9 and Th2 cells and levels of IL-9, IL-4, IL-10, and TGF- β were also significantly decreased in PCE group. In conclusion, the ratios of Th9 cells and IL-9 levels were significantly decreased after treatment, suggesting that Th9/IL-9 may be involved in immune response induced by Echinococcus granulosus infection.
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Authors | Nannan Pang, Fengbo Zhang, Xiumin Ma, Zhaoxia Zhang, Hui Zhao, Yan Xin, Song Wang, Yuejie Zhu, Hao Wen, Jianbing Ding |
Journal | Mediators of inflammation
(Mediators Inflamm)
Vol. 2014
Pg. 781649
( 2014)
ISSN: 1466-1861 [Electronic] United States |
PMID | 24799769
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- GATA3 Transcription Factor
- Interleukin-9
- Proto-Oncogene Proteins
- RNA, Messenger
- Trans-Activators
- Transforming Growth Factor beta
- proto-oncogene protein Spi-1
- Interleukin-10
- Interleukin-4
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Topics |
- Animals
- Echinococcosis
(immunology, metabolism)
- Echinococcus granulosus
(immunology, pathogenicity)
- GATA3 Transcription Factor
(genetics, metabolism)
- Humans
- Interleukin-10
(genetics, metabolism)
- Interleukin-4
(genetics, metabolism)
- Interleukin-9
(genetics, metabolism)
- Proto-Oncogene Proteins
(genetics, metabolism)
- RNA, Messenger
(genetics)
- Th2 Cells
- Trans-Activators
(genetics, metabolism)
- Transforming Growth Factor beta
(genetics, metabolism)
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