Grape seed pro-anthocyanidins ameliorates radiation-induced lung injury.

Abstract

Radiation-induced lung injury (RILI) is a potentially fatal and dose-limiting complication of thoracic radiotherapy. This study was to investigate the protective effects of grape seed pro-anthocyanidins (GSPs), an efficient antioxidant and anti-carcinogenic agent, on RILI. In our study, it was demonstrated that acute and late RILI was ameliorated after GSPs treatment possibly through suppressing TGF-β1/Smad3/Snail signalling pathway and modulating the levels of cytokines (interferon-γ, IL-4 and IL-13) derived from Th1/Th2 cells. In addition, a sustained high level of PGE2 was also maintained by GSPs treatment to limited fibroblast functions. As shown by electron spin resonance spectrometry, GSPs could scavenge hydroxyl radical (•OH) in a dose-dependent manner, which might account for the mitigation of lipid peroxidation and consequent apoptosis of lung cells. In vitro, GSPs radiosensitized lung cancer cell A549 while mitigating radiation injury on normal alveolar epithelial cell RLE-6TN. In conclusion, the results showed that GSPs protects mice from RILI through scavenging free radicals and modulating RILI-associated cytokines, suggesting GSPs as a novel protective agent in RILI.

• Authors: Yijuan Huang, Wen Liu, Hu Liu, Yanyong Yang, Jianguo Cui, Pei Zhang, Hainan Zhao, Feng He, Ying Cheng, Jin Ni, Jianming Cai, Bailong Li, Fu Gao
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 18 Issue 7 Pg. 1267-77 (Jul 2014) ISSN: 1582-4934 [Electronic] England
• PMID: 24758615 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Chemical References

• Cytokines
• Plant Extracts
• RNA, Messenger
• Radiation-Protective Agents

Topics

• Animals
• Blotting, Western
• Cytokines (genetics, metabolism)
• Electron Spin Resonance Spectroscopy
• Female
• Gamma Rays (adverse effects)
• Humans
• Immunoenzyme Techniques
• Lung Injury (etiology, metabolism, prevention & control)
• Mice
• Mice, Inbred C57BL
• Plant Extracts (pharmacology)
• RNA, Messenger (genetics)
• Radiation Injuries, Experimental (etiology, metabolism, prevention & control)
• Radiation-Protective Agents (pharmacology)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Seeds (chemistry)
• Vitis (chemistry)