Abstract | BACKGROUND: It is well understood that epithelial mesenchymal transformation occurs when retinal pigment epithelial cells, sourced from either a cell line or cadaver eye, are cultured in the presence of cadaver-derived vitreous. We sought to study the changes in retinal pigment epithelial cells when cell line-derived retinal pigment epithelial cells are cultured in the presence of pathological vitreous. DESIGN: Prospective study. SAMPLES: METHODS: D407 retinal pigment epithelial cells were cultured in the presence of cadaver-derived vitreous or vitreous/subretinal fluid derived from patients undergoing retinal reattachment surgeries. Besides the changes in phenotypic characteristics, the viability, proliferation, migration, mesenchymal marker expression and changes in the extracellular matrix components were also evaluated. MAIN OUTCOME MEASURES: Fibrotic phenotype in cell culture. RESULTS: CONCLUSIONS: We propose that it is the synergistic effect of the combined use of (i) pathological vitreous, rather than cadaver-derived vitreous (since rhegmatogenous retinal detachment-derived pathological vitreous and subretinal fluid contain exaggerated amounts of growth factors, which could predispose to proliferative vitreoretinopathy development) and (ii) cells from an immortal cell culture (cell line), rather than from primary cell cultures (since cells subjected to continuous serial passaging acquire some mesenchymal characteristics), which together result in not only a unique phenotype, but also prime these cells towards display of features associated with proliferative vitreoretinopathy.
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Authors | Maryada Sharma, Anil Tiwari, Shweta Sharma, Reema Bansal, Vishali Gupta, Amod Gupta, Manni Luthra-Guptasarma |
Journal | Clinical & experimental ophthalmology
(Clin Exp Ophthalmol)
Vol. 42
Issue 8
Pg. 745-60
(Nov 2014)
ISSN: 1442-9071 [Electronic] Australia |
PMID | 24612444
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 Royal Australian and New Zealand College of Ophthalmologists. |
Chemical References |
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Biomarkers
(metabolism)
- Cell Movement
- Cell Proliferation
- Cell Survival
- Cells, Cultured
- Child
- Child, Preschool
- Fluorescent Antibody Technique, Indirect
- Humans
- Infant
- Middle Aged
- Phenotype
- Prospective Studies
- Retinal Detachment
(surgery)
- Retinal Pigment Epithelium
(metabolism, pathology)
- Subretinal Fluid
(physiology)
- Tissue Donors
- Vitreoretinopathy, Proliferative
(metabolism, pathology)
- Vitreous Body
(pathology)
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