Profiling of autoantibodies in sera of pancreatic cancer patients.

Abstract

BACKGROUND:

Although autoantibodies to cancer antigens are candidates for biomarkers, no comprehensive studies to detect cancer-specific antibodies have been performed. This study identified autoantibodies in the sera of pancreatic cancer (PC) patients using proteomics based on a wheat germ cell-free protein production system.

METHODS:

We constructed a biotinylated protein library of 2,183 genes. Interactions between biotinylated proteins and serum antibodies were detected by AlphaScreen® assay. Relative luminescence signals of each protein in 37 PC patients and 20 healthy controls were measured, and their sensitivity and specificity for PC were calculated.

RESULTS:

Luminescence signals of nine proteins were significantly higher than those of healthy controls, with calcium and integrin binding 1 (CIB1) protein showing the greatest significance (p = 0.002). Sensitivity, specificity, positive predictive value and negative predictive value of CIB1 autoantibody alone for PC were 76, 70, 82, and 61 %, respectively, and 97, 35, 74, and 88 %, respectively, when the four most significant proteins were combined. Presence of these autoantibodies did not vary significantly with other clinicopathological characteristics.

CONCLUSION:

Several autoantibodies, including CIB1, are potential biomarkers for PC.

• Authors: Yosuke Nagayoshi, Masafumi Nakamura, Kazuhiro Matsuoka, Takao Ohtsuka, Yasuhisa Mori, Hiroshi Kono, Teppei Aso, Noboru Ideno, Shunichi Takahata, Akihide Ryo, Hiroyuki Takeda, Tetsuhide Ito, Yoshinao Oda, Yaeta Endo, Tatsuya Sawasaki, Masao Tanaka
• Journal: Annals of surgical oncology (Ann Surg Oncol) Vol. 21 Suppl 3 Pg. S459-65 (Jun 2014) ISSN: 1534-4681 [Electronic] United States
• PMID: 24585405 (Publication Type: Comparative Study, Journal Article, Randomized Controlled Trial)

Chemical References

• Antigens, Neoplasm
• Autoantibodies
• Biomarkers, Tumor
• CIB1 protein, human
• Calcium-Binding Proteins

Topics

• Adult
• Aged
• Antigens, Neoplasm (immunology)
• Autoantibodies (blood)
• Biomarkers, Tumor (blood)
• Calcium-Binding Proteins (blood)
• Case-Control Studies
• Enzyme-Linked Immunosorbent Assay
• Female
• Follow-Up Studies
• Humans
• Male
• Middle Aged
• Pancreatic Neoplasms (blood, diagnosis, immunology)
• Prognosis
• Sensitivity and Specificity