MiRNA-145 is involved in the development of resistin-induced insulin resistance in HepG2 cells.

Resistin is associated with insulin resistance, and determining its developmental and molecular mechanisms may help the development of novel treatments. MicroRNAs (miRNAs) are involved in many physiological and pathological processes as negative regulators. However, it remains unclear whether miRNAs play a role in resistin-induced insulin resistance. We performed mouse liver miRNA microarrays to analyze the differences in expression between resistin-treated and control mice. Resistin upregulated miR-145 both in vivo and in vitro. Therefore, we aimed to study whether miR-145 played a role in resistin-induced insulin resistance.
We transfected HepG2 cells, and used miR-145 mimics and inhibitors to assess the role of miR-145 in resistin-induced insulin resistance. The overexpression of miR-145 inhibited glucose uptake in HepG2 cells, diminished the phosphorylation of Akt and IRS-1, and induced insulin resistance in hepatocytes. Next, a study of transcriptional regulation revealed that p65 was essential for the upregulation of miR-145 by resistin, and chromatin immunoprecipitation (ChIP) confirmed that p65 could bind to the promoter region of miR-145.
miR-145 plays a role in the development of resistin-induced insulin resistance via the p65 pathway.
AuthorsFengyun Wen, Yi Yang, Dan Jin, Jun Sun, Xiaoling Yu, Zaiqing Yang
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 445 Issue 2 Pg. 517-23 (Mar 7 2014) ISSN: 1090-2104 [Electronic] United States
PMID24548410 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier Inc. All rights reserved.
Chemical References
  • IRS1 protein, human
  • IRS2 protein, human
  • Insulin Receptor Substrate Proteins
  • MIRN145 microRNA, human
  • MicroRNAs
  • Resistin
  • Transcription Factor RelA
  • Glucose
  • Animals
  • Down-Regulation
  • Glucose (metabolism)
  • HEK293 Cells
  • Hep G2 Cells (metabolism, pathology)
  • Humans
  • Insulin Receptor Substrate Proteins (genetics)
  • Insulin Resistance (genetics, physiology)
  • Male
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs (genetics)
  • Promoter Regions, Genetic
  • Resistin (metabolism)
  • Transcription Factor RelA (metabolism)
  • Up-Regulation

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: