Amelioration of autoimmune arthritis by naringin through modulation of T regulatory cells and Th1/Th2 cytokines.

Abstract

Naringin, a well-known flavanone glycoside found in grapefruit and other citrus fruits, was determined to be an effective anti-inflammatory compound. We investigated the effect of naringin on the key mediators of arthritic inflammation, namely T cell subsets, CD4(+)GITR(+) expressing cells, CD4(+)CD25(+)Foxp3(+) (Treg), Th1/Th2 cytokines and inflammatory mediators. We treated Balb/c mice (p.o.) with naringin (20, 40 and 80 mg/kg) for 14 days. Compared with the vehicle-treated and arthritic-control mice, the naringin treatment demonstrated a considerable decrease in the level of T cells, CD4(+)GITR(+), Th1 cytokine and inflammatory mediator expressions. In contrast, naringin treatment resulted in significantly up-regulated Treg and Th2 cytokine levels. Therefore, the naringin-induced inhibition of the T cells, various pro-inflammatory cytokines and inflammatory mediators that facilitate cellular infiltration into the joints might have contributed to its anti-arthritic activity. Our data suggest that naringin diminished the AIA in mice and it could be a potential alternative/adjunct treatment for RA.

• Authors: Sheikh Fayaz Ahmad, Khairy M A Zoheir, Hala E Abdel-Hamied, Abdelkader E Ashour, Saleh A Bakheet, Sabry M Attia, Adel R A Abd-Allah
• Journal: Cellular immunology (Cell Immunol) Vol. 287 Issue 2 Pg. 112-20 (Feb 2014) ISSN: 1090-2163 [Electronic] Netherlands
• PMID: 24487035 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• CD4 Antigens
• Cytokines
• Flavanones
• Forkhead Transcription Factors
• Foxp3 protein, mouse
• Glucocorticoid-Induced TNFR-Related Protein
• Inflammation Mediators
• Interleukin-2 Receptor alpha Subunit
• Tnfrsf18 protein, mouse
• naringin

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (therapeutic use)
• Arthritis (immunology, therapy)
• Autoimmune Diseases (immunology, therapy)
• CD4 Antigens (metabolism)
• Cells, Cultured
• Citrus paradisi (chemistry)
• Cytokines (metabolism)
• Disease Progression
• Female
• Flavanones (therapeutic use)
• Forkhead Transcription Factors (metabolism)
• Glucocorticoid-Induced TNFR-Related Protein (metabolism)
• Inflammation Mediators (metabolism)
• Interleukin-2 Receptor alpha Subunit (metabolism)
• Mice
• Mice, Inbred BALB C
• T-Lymphocyte Subsets (drug effects, immunology)
• T-Lymphocytes, Regulatory (drug effects, immunology)
• Th1-Th2 Balance