Iron availability is increased in individual human ovarian follicles in close proximity to an endometrioma compared with distal ones.

Abstract

STUDY QUESTION:

Does the iron content of an endometrioma represent a potential source of toxicity for the adjacent follicles?

SUMMARY ANSWER:

The presence of an endometrioma increases iron and H/L ferritin levels, and transferrin receptor (TfR1) mRNA in individual follicles proximal to the endometrioma and is accompanied by reduced oocyte retrieval.

WHAT IS KNOWN ALREADY:

Levels of free iron in endometriotic ovarian cysts are much higher than those in normal serum or in non-endometriotic ovarian cysts. The presence of an endometrioma exerts a detrimental effect on the surrounding healthy ovarian tissue as reflected by a reduced number of developing follicles and oocytes retrieved in IVF cycles.

STUDY DESIGN, SIZE, DURATION:

This is a research study with prospective collection and evaluation of individual follicles (follicular fluid and luteinized granulosa cells) from the affected and the healthy ovaries of 13 women with unilateral endometrioma.

PARTICIPANTS/MATERIALS, SETTING, METHODS:

Individual follicular samples (145) were obtained from 13 women with endometriosis-related infertility undergoing IVF-ICSI procedures from May 2012 to March 2013. All women had unilateral endometrioma not previously treated with surgery; the contralateral ovary was free of endometriomas and previous surgery. The average ± SEM age was 35.36 ± 2.5 years with anti-Mullerian hormone levels of 2.03 ± 0.55 ng/ml. Follicles were classified as: (i) proximal follicles, in physical contact with the endometrioma; (ii) distal follicles, present in the affected ovary but not in close contact with the endometrioma and (iii) contralateral follicles, in the contralateral healthy ovary. Iron content was measured by the FerroZine method. H/L ferritin subunits were evaluated by specific enzyme-linked immunosorbant assays. Expression of H ferritin and TfR1 was examined by semi-quantitative RT-PCR. Oocyte retrieval rates and Day 3 embryo quality were analyzed.

MAIN RESULTS AND THE ROLE OF CHANCE:

Total iron levels were higher in endometrioma-proximal follicles compared with endometrioma-distal ones (P = 0.009) and to follicles in the healthy ovary (P = 0.02). L ferritin was higher in proximal versus distal follicles (P = 0.044) or follicles from the healthy ovary (P = 0.027). H ferritin was higher in the proximal and distal follicles compared with follicles in the healthy ovary (P = 0.042 and P = 0.0067, respectively). H ferritin transcript levels in granulosa cells were higher in proximal follicles versus follicles from healthy ovary (P = 0.02). TfR1 transcript levels were higher in proximal versus distal follicles (P = 0.03) and versus follicles from the healthy ovary (P = 0.04). The oocyte retrieval rate was lower in proximal and distal follicles than in follicles from the healthy ovary (P = 0.001 and P = 0.04, respectively).

LIMITATIONS, REASONS FOR CAUTION:

This is a study on a relatively small sample size and confirmation in a larger group of patients may be required. The method used to purify luteinized granulosa cells offers the best combination of purity, viability and total number of cells recovered. However, a minor contamination by CD45(+) cells (<5%) cannot be excluded.

WIDER IMPLICATIONS OF THE FINDINGS:

This study represents a further in-depth analysis of the toxic influence of the endometrioma content on the surrounding follicles. We demonstrate the presence of iron-related compounds that are potentially toxic to developing ovarian follicles adjacent to the endometrioma during IVF. Our findings provide novel information that suggests that when surgical removal of the endometrioma is not the option, follicle aspiration at sites distant from the endometrioma might increase the probability of retrieving oocytes.

STUDY FUNDING/COMPETING INTEREST(S):

This project was supported by Fondazione Giorgio Pardi, Milan, Italy. The authors have no competing financial interests in relation to the content of this research paper.

TRIAL REGISTRATION NUMBER:

NA.

• Authors: Ana Maria Sanchez, Enrico Papaleo, Laura Corti, Paolo Santambrogio, Sonia Levi, Paola Viganò, Massimo Candiani, Paola Panina-Bordignon
• Journal: Human reproduction (Oxford, England) (Hum Reprod) Vol. 29 Issue 3 Pg. 577-83 (Mar 2014) ISSN: 1460-2350 [Electronic] England
• PMID: 24430779 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• CD71 antigen
• Receptors, Transferrin
• Ferritins
• Iron

Topics

• Antigens, CD (biosynthesis)
• Endometriosis (surgery)
• Female
• Ferritins (biosynthesis)
• Granulosa Cells (metabolism)
• Humans
• Iron (metabolism)
• Oocyte Retrieval
• Ovarian Follicle (drug effects, metabolism)
• Receptors, Transferrin (biosynthesis)