Abstract |
We show that minor capsid protein L2 is full length in clinical virion isolates and prepare furin-cleaved pseudovirus (fcPsV) as a model of the infectious intermediate for multiple human papillomavirus (HPV) types. These fcPsV do not require furin for in vitro infection, and are fully infectious in vivo. Both the γ- secretase inhibitor XXI and carrageenan block fcPsV infection in vitro and in vivo implying that they act after furin-cleavage of L2. Despite their enhanced exposure of L2 epitopes, vaccination with fcPsV particles fails to induce L2 antibody, although L1-specific responses are similar to PsV with intact L2. FcPsV can be applied in a simple, high-throughput neutralization assay that detects L2-specific neutralizing antibodies with >10-fold enhanced sensitivity compared with the PsV-based assay. The PsV and fcPsV-based assays exhibit similar sensitivity for type-specific antibodies elicited by L1 virus-like particles (VLP), but the latter improves detection of L1-specific cross-type neutralizing antibodies.
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Authors | Joshua W Wang, Subhashini Jagu, Kihyuck Kwak, Chenguang Wang, Shiwen Peng, Reinhard Kirnbauer, Richard B S Roden |
Journal | Virology
(Virology)
Vol. 449
Pg. 304-16
(Jan 20 2014)
ISSN: 1096-0341 [Electronic] United States |
PMID | 24418565
(Publication Type: Journal Article)
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Copyright | © 2013 Published by Elsevier Inc. |
Chemical References |
- Antibodies, Neutralizing
- Antibodies, Viral
- Capsid Proteins
- Papillomavirus Vaccines
- Furin
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Topics |
- Alphapapillomavirus
(genetics, immunology, physiology)
- Animals
- Antibodies, Neutralizing
(immunology)
- Antibodies, Viral
(immunology)
- Capsid Proteins
(administration & dosage, genetics, immunology)
- Cross Reactions
- Furin
(metabolism)
- Humans
- Mice, Inbred BALB C
- Neutralization Tests
- Papillomavirus Infections
(immunology, prevention & control, virology)
- Papillomavirus Vaccines
(administration & dosage, genetics, immunology)
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