Abstract | BACKGROUND: Schizophyllum commune is one of the causative agents of basidiomycosis including disorders such as allergic bronchopulmonary mycosis, allergic fungal sinusitis, and mucoid impaction of bronchi, the incidence of those of which has been increasing. These mycoses are difficult to diagnose because only a limited number of diagnostic tools are currently available. The biggest problem is that no specific antigens of S. commune have been identified to enable serodiagnosis of the disease. OBJECTIVE: In this study, we attempted to identify a major antigen of S. commune to establish a reliable serodiagnostic method. METHODS: RESULTS: The protein identified as a major antigen of S. commune was named Sch c 1; it was a homolog of glucoamylase. The IgG and IgE titres against Sch c 1 in patient sera were significantly higher than those in healthy volunteer sera (P < 0.01). CONCLUSIONS AND CLINICAL RELEVANCE: Sch c 1 is recognized by the host immune system of patients as an antigen/ allergen. The purified glucoamylase Sch c 1 is a promising candidate antigen for the serodiagnosis of S. commune-induced mycosis.
|
Authors | T Toyotome, M Satoh, M Yahiro, A Watanabe, F Nomura, K Kamei |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 44
Issue 3
Pg. 450-7
(Mar 2014)
ISSN: 1365-2222 [Electronic] England |
PMID | 24372664
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2013 John Wiley & Sons Ltd. |
Chemical References |
- Allergens
- Antibodies, Fungal
- Antigens, Fungal
- Immunoglobulin G
- Immunoglobulin E
- Glucan 1,4-alpha-Glucosidase
|
Topics |
- Allergens
(chemistry, immunology)
- Amino Acid Sequence
- Antibodies, Fungal
(blood, immunology)
- Antigens, Fungal
(chemistry, immunology)
- Cross Reactions
(immunology)
- Glucan 1,4-alpha-Glucosidase
(chemistry, immunology)
- Humans
- Immunoglobulin E
(blood, immunology)
- Immunoglobulin G
(blood, immunology)
- Molecular Sequence Data
- Mycoses
(blood, immunology)
- Pulmonary Aspergillosis
(immunology)
- Schizophyllum
(enzymology, immunology)
- Sequence Alignment
|