Abstract | OBJECTIVE: To investigate the effects of oxygen on the proliferation and angiogenesis of endometriosis in vivo. DESIGN: Animal studies. SETTING: Animal research facility. ANIMAL(S): INTERVENTION(S): Human eutopic endometrial tissues were randomized to normoxia, hyperoxia, or hypoxia pretreatment and were subcutaneously implanted into estrogen-treated ovariectomized SCID mice. MAIN OUTCOME MEASURE(S): The growth and quality of the implants were measured, and the expression of proliferation- and angiogenesis-associated markers (i.e., Ki67, CD31, vascular endothelial growth factor, and hypoxia-inducible factor-1α) were assessed using immunohistochemistry and Western blot analyses. RESULT(S): The growth curves of the implants were distinct with different oxygen pretreatments. The growth of the implants of the hypoxia group was significantly increased compared with the normoxia group, but the growth of the implants of the hyperoxia group was significantly decreased compared with the normoxia group. Microscopic examination indicated that lesions with hyperplastic cylindrical glandular epithelium were surrounded by the endometrial stroma in the hypoxia group, but the glandular epithelium was partially depauperate in the hyperoxia group. The expression of Ki67, CD31, vascular endothelial growth factor, and hypoxia-inducible factor-1α in the hypoxia-pretreated implants was significantly higher compared with the hyperoxia or normoxia groups. CONCLUSION(S):
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Authors | Zechun Lu, Weiyi Zhang, Shufang Jiang, Jie Zou, Yali Li |
Journal | Fertility and sterility
(Fertil Steril)
Vol. 101
Issue 2
Pg. 568-76
(Feb 2014)
ISSN: 1556-5653 [Electronic] United States |
PMID | 24290003
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Angiogenesis Inhibitors
- Oxygen
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Topics |
- Adult
- Angiogenesis Inhibitors
(administration & dosage)
- Animals
- Cell Proliferation
- Endometriosis
(genetics, pathology, therapy)
- Female
- Heterografts
(blood supply, pathology)
- Humans
- Hyperoxia
(genetics, pathology, therapy)
- Hypoxia
(genetics, pathology, therapy)
- Mice
- Mice, SCID
- Middle Aged
- Neovascularization, Pathologic
(genetics, pathology, therapy)
- Oxygen
(administration & dosage)
- Random Allocation
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