Abstract | BACKGROUND: MATERIALS AND METHODS: The study covered 92 patients with primary brain tumors and 92 age-matched healthy control subjects. Evaluation of β3 integrin (Leu33Pro (rs5918)) and ICAM-1 (R241G (rs1799969) and K469E (rs5498)) gene polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: According to results of our research, the A allele of the ICAM-1 R241G gene polymorphism appeared to be a risk factor for primary brain tumors (p<0.001). Similarly, the frequency of the A mutant allele of ICAM-1 R241G was statistically significant in patients with brain tumors classified as glioma (p<0.001). When allele and genotype distributions of ICAM- 1 K469E, ICAM-1 R241G and β3 integrin Leu33Pro gene polymorphisms were evaluated with age, sex, and smoking, there were no statistically significant differences. Haplotype analysis revealed that the frequencies of GAC (rs1799969-rs5498-rs5918) and GAT (rs1799969-rs5498-rs5918) haplotypes were significantly lower in patients as compared with controls (p=0.001; p=0.036 respectively). CONCLUSIONS: This study provides the first evidence that ICAM-1 R241G SNP significantly contributes to the risk of primary brain tumors in a Turkish population. In addition, our results suggest that ICAM-1 R241G in combination ICAM-1 K469E may have protective effects against the development of brain cancer.
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Authors | Umit Yilmaz, Umit Zeybek, Ozlem Timirci Kahraman, Ali Metin Kafadar, Bahar Toptas, Nesibe Yamak, Faruk Celik, Ilhan Yaylim |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 14
Issue 10
Pg. 5929-34
( 2013)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 24289603
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Biomarkers, Tumor
- ITGB3 protein, human
- Integrin beta3
- Intercellular Adhesion Molecule-1
- DNA
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Topics |
- Biomarkers, Tumor
(genetics)
- Brain Neoplasms
(genetics)
- Case-Control Studies
- DNA
(analysis, genetics)
- Female
- Follow-Up Studies
- Genotype
- Haplotypes
(genetics)
- Humans
- Integrin beta3
(genetics)
- Intercellular Adhesion Molecule-1
(genetics)
- Male
- Middle Aged
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single Nucleotide
(genetics)
- Prognosis
- Prospective Studies
- Risk Factors
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